2022 Fiscal Year Annual Research Report
Development of a novel hydrogel for in situ Laser-Assisted Bioprinting to promote bone regeneration
| Project/Area Number |
22F32101
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| Research Institution | Kyoto University |
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Principal Investigator |
田畑 泰彦 京都大学, 医生物学研究所, 教授 (50211371)
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| Co-Investigator(Kenkyū-buntansha) |
KEROUREDAN OLIVIA 京都大学, 医生物学研究所, 外国人特別研究員
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| Project Period (FY) |
2022-07-27 – 2023-03-31
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| Keywords | tissue engineering / bone regeneration / bioactive glass / coculture / vascularization / osteogenesis / bioprinting / biofabrication |
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| Outline of Annual Research Achievements |
Gelatin (Gel5wt%)-sheets incorporating bioactive glasses (BG0wt%, 1wt%, 3wt% or 5wt%) were fabricated and underwent a dehydrothermal crosslinking (CL120°C, CL140°C or CL160°C 24h). The condition “Gel5wt% BG1wt% CL160°C” allowed to obtain the best results in terms of physical (porosity, pore size, swelling ratio), mechanical (Young modulus) and biological (viability, proliferation, ALP activity, calcium deposition) properties, in monoculture and coculture models. After immersion into simulated body fluid, Ca/P molar ratios measured using EDX analysis were between 1.72 and 1.77, suggesting a good bioactivity of the biomaterial. In coculture, this formulation supported the formation of microvascular networks. Finally, this novel biopaper was successfully used as a receiving substrate for Laser-Assisted Bioprinting technology, but also adapted to 3D-moulding and stacking approaches to generate 3D constructs.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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