2023 Fiscal Year Research-status Report
Rewiring the brain: remote-controlled axon guidance by magnetic nanoparticles to improve Parkinson's therapies
| Project/Area Number |
22K06430
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
土井 大輔 京都大学, iPS細胞研究所, 特定拠点講師 (10587851)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Keywords | Parkinson's Disease / Magnetic Nanoparticle / Cell Replacment / Stem Cells / Dopaminergic Neurons |
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| Outline of Annual Research Achievements |
In the FY2023, large efforts were made towards the magnetic manipulation of nanoparticle-loaded dopaminergic neurospheres. In close collaboration with the Raffa Lab (Pisa, Italy) we were able to show the asymmetric neurite growth in direction of the magnetic field.
As the transition to animal models requires further optimization, we performed the magnetic manipulation in ex vivo cultures. These cultures are 350 um thick sections of the mouse brain. Nanoparticle=loaded spheres were grafted into these ex vivo cultures. We were able to see neurite growth in the direction of the magnetic field.
In summary, in the FY2023, we were able to translate our approach to the neurosphere model and were able to show that the stretch growth is still presence in an organotypic environment.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
As mentioned above, we were able to tackle the two major points proposed for FY2023. On the one hand, the magnetic manipulation of neurospheres was done. On the other hand, the first step towards the application of our approach in vivo was tested. Now, the actual application in vivo can be tested.
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| Strategy for Future Research Activity |
The first step is generating more data with our neurosphere and ex vivo ex vivo model. After optimizing the parameters, we can translate the approach to the animal model. Here, the biggest task is to finalize designing an appropriate magnetic applicator.
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| Causes of Carryover |
The animal experiments are scheduled for the upcoming fiscal year and therefore more costs will be generated. Budget is carried over to cover these costs.
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