2022 Fiscal Year Research-status Report
Analyses of in vivo neuroprotective role of STB/HAP1 against autonomic and motoneuron degeneration in the central and peripheral nervous system
| Project/Area Number |
22K06792
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| Research Institution | Yamaguchi University |
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Principal Investigator |
イスラム エムディノビウル 山口大学, 大学院医学系研究科, 講師 (80759671)
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| Co-Investigator(Kenkyū-buntansha) |
篠田 晃 山口大学, 大学院医学系研究科, 教授 (40192108)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Keywords | HAP1 / Stigmoid body / Motor neuron / Autonomic neuron |
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| Outline of Annual Research Achievements |
In the first year of our study, we clarified the neuroanatomical distribution of STB/HAP1 in the brainstem of adult mice. We also elucidated the relationships of STB/HAP1 with ChAT, with particular emphasis on autonomic and motor neurons. Our results suggest that cranial nerve motor nuclei might be more vulnerable to neurodegenerative stresses than STB/HAP1-expressing brainstem nuclei. In addition, we clarified the STB/HAP1 expression in the enteric and tongue autonomic ganglia.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have completed our first objective, which includes the relationships of STB/HAP1 with ChAT in the brainstem. We have published our data in peer-reviewed journals.
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| Strategy for Future Research Activity |
1. We will clarify the immunohistochemical relationships of STB/HAP1 with the causal agents of certain neurodegenerative diseases in vivo.
2. We will examine STB/HAP1-expression in autonomic ganglion and determine the autonomic neuron number changes in the CNS/PNS of HAP1-KO mice. Furthermore, we will compare autonomic function-related behavioral test batteries between wild-type and HAP1-KO mice.
3. Aged mouse is an excellent model to examine the vulnerability to motor neuron degeneration. We will also determine the changes in motor neuron number motor function-related behavioral test battery comparison between aged-wild type and aged-HAP1-TG mice.
4. The microbiota/gut-and-brain axis is an emerging and widely accepted concept. STB/HAP1 might affect microbiota colonization in the intestine, jeopardizing the neurodegeneration in ENS and CNS. Using HAP1-KO mice, we will examine the effects of STB/HAP1 on enteric neurons and the composition of gut microbiota.
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| Causes of Carryover |
There was a plan to participate in the international conference in the USA; however, due to the COVID-19 pandemic, we could not attend in person. The incurring amount of money will be used for the experiment to clarify the immunohistochemical relationships of STB/HAP1 with the causal agents of certain neurodegenerative diseases in vivo in this fiscal year. There is also a plan to use this money to pay the color charge of articles in peer-reviewed journals.
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