2023 Fiscal Year Research-status Report
Analyses of in vivo neuroprotective role of STB/HAP1 against autonomic and motoneuron degeneration in the central and peripheral nervous system
| Project/Area Number |
22K06792
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
イスラム エムディノビウル 山口大学, 大学院医学系研究科, 講師 (80759671)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
篠田 晃 山口大学, 大学院医学系研究科, 教授 (40192108)
|
|---|
| Project Period (FY) |
2022-04-01 – 2025-03-31
|
| Keywords | HAP1 / Neuroprotection / ChAT / Huntingtin / STB |
|---|
| Outline of Annual Research Achievements |
In the first year, we clarified the relationships of STB/HAP1 with ChAT in the brainstem, particularly in motoneurons and autonomic neurons. In the second year, we further investigated the relationships of STB/HAP1 with ChAT in the forebrain. We found that the cholinergic neurons of MS, VDB, and LS that are mainly projected to the hippocampus and parahippocampal gyrus might be protected by STB/HAP1 to regulate the sensory-motor integration or cognition during neurodegenerative/psychotic stresses. In contrast, the STB/HAP1 and ChAT co-expression devoid area (e.g., CPU and cortex) might be more prone to neurodegeneration. In addition, we also clarified that HAP1 is highly expressed in the submucosal plexuses of the enteric nervous system and the lingual Ganglia.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have completed objectives 1 and 2 of our current research, and the other objectives are progressing smoothly. We have presented our research findings at various regional, national, and international conferences. We have also published two articles in peer-reviewed international journals.
|
| Strategy for Future Research Activity |
1. We will clarify the immunohistochemical relationships of STB/HAP1 with the Huntingtin in the brain and spinal cord.
2. We will clarify the immunohistochemical relationships of STB/HAP1 with the Serotonin in the brain.
3. We will examine STB/HAP1-expression in autonomic ganglion and determine the autonomic neuron number changes in the CNS/PNS of HAP1-KO mice. Furthermore, we will compare autonomic function-related behavioral test batteries between wild-type and HAP1-KO mice.
4. The aged mouse is an excellent model for examining motor neuron degeneration vulnerability. We will also determine the changes in motor neuron number and motor function-related behavioral test battery comparison between aged-wild type and aged-HAP1-TG mice.
|
| Causes of Carryover |
We were planning to attend international academic conferences in the USA. However, we had a Japanese conference at the same time, so we could not participate in that conference. In addition, we needed a publication charge for a research paper in the third year of this research project, so we kept some money to be used in the next fiscal year.
|
