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2023 Fiscal Year Research-status Report

Clot wave analysis with platelet- Study on platelet role for coagulation abnormality

Research Project

Project/Area Number 22K07441
Research InstitutionMie University

Principal Investigator

和田 英夫  三重大学, 医学系研究科, リサーチアソシエイト (40158704)

Project Period (FY) 2022-04-01 – 2026-03-31
Keywords凝固波形(CWA) / 血小板 / 凝固異常 / sCLEC-2 / sTF/FIXa / 癌 / 血友病 / 脳梗塞
Outline of Annual Research Achievements

・播種性血管内凝固(DIC)診断に、super formula sCLEC-2 (soluble C-type lectin-like receptor 2 (sCLEC-2)x D-dimer/血小板数が、厚生省DIC診断基準によるDICと最も有意に一致した(Diagnostics, Basel, 2023; 13: 2299)。
・急性脳梗塞の診断にはsCLEC-2 x D-dimerが最も有用であり、sCLEC-2/D-dimerは心原生脳梗塞で有意に低下し、アテローム性脳梗塞で有意に増加した(Clin Appl Thromb Hemost, 2024; 30: 10760296241232858)。sCLEC-2 x D-dimerは血栓症の診断に、sCLEC-2/D-dimerは動脈性と静脈性の血栓の鑑別に、sCLEC-2 x D-dimer/血小板数は微小血栓の診断に有用であった。
・膵癌ならびに肝癌などの担癌患者の凝固波形(CWA)は、CWA-活性化部分トロンボプラスチン時間(APTT)ならびにCWA-small amount of tissue factor induced FIX activation (sTF/FIXa)のpeak height (PH)が有意に高値であり、過凝固状態の診断に有用であった。
・CWA-APTTやCWA-sTF/FIXaのPeak time (PT)xPHあるいはPH/PTは、過凝固状態や血栓症の予知に有用であった(Ann Hematol Onco, 2024, Clin Appl Thromb Hemost, 2024; in press)。過凝固状態の原因はthrombin burstによることを、CWA-thrombin time(time)により証明した。
・FVIII製剤を投与されている血友病患者では、CWA-sTF/FIXaのPT短縮、PH増加がみられ、過凝固状態が示唆された(J Clin Med, 2023; 12: 6320)。同様に、過凝固状態の原因はthrombin burstによることを、CWA-thrombin time(time)により証明した。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

・脳梗塞、COVID-19、心筋梗塞例、担癌患者などの症例は充分集積できた。
・大出血例や担癌患者血栓症の発症例は、まだ症例数が足りない。
・CWA-APTT、CWA-sTF/FIXa、CWA-TT、sCLEC-2の測定法が順調に行えた。
・過去の実験実績があり、測定法が簡便で、比較的多数例を測定できた。
・Emicizumabや中和抗体などの試薬が入手できた。 ・

Strategy for Future Research Activity

・心筋梗塞や脳梗塞症例について発症時ならびに発症後の経日変化の解析を進める。
・抗血小板療法や抗凝固療法との関係を示す。
・血友病例を含めた大出血例の解析を進める。
・血小板活性化剤によるCWAへの影響を検討する。
・血栓予防への適応を検討する。

  • Research Products

    (22 results)

All 2024 2023

All Journal Article (18 results) (of which Int'l Joint Research: 18 results,  Peer Reviewed: 18 results,  Open Access: 14 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results)

  • [Journal Article] Super Formula for Soluble C-Type Lectin-Like Receptor 2?×?D-Dimer in Patients With Acute Cerebral Infarction2024

    • Author(s)
      Kamon Toshitaka、Wada Hideo、Horie Shotaro、Inaba Tomoya、Okamoto Karin、Shiraki Katsuya、Ichikawa Yuhuko、Ezaki Minoru、Shimaoka Motomu、Nishigaki Akisato、Shindo Akihiro、Shimpo Hideto、Ito Nobuo
    • Journal Title

      Clinical and Applied Thrombosis/Hemostasis

      Volume: 30 Pages: ー

    • DOI

      10.1177/10760296241232858

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A systematic review and meta-analysis of recombinant human soluble thrombomodulin for the treatment of DIC associated with hematological malignancies2024

    • Author(s)
      Kawano Noriaki、Fukatsu Masahiko、Yamakawa Kazuma、Seki Yoshinobu、Wada Hideo、Okamoto Kohji、Ikezoe Takayuki
    • Journal Title

      International Journal of Hematology

      Volume: 119 Pages: 416~425

    • DOI

      10.1007/s12185-023-03704-w

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association of D-dimer level with thrombotic events, bleeding, and mortality in Japanese patients with solid tumors: a Cancer-VTE Registry subanalysis2024

    • Author(s)
      Nakamura Mashio、Sakon Masato、Sasako Mitsuru、Okusaka Takuji、Mukai Hirofumi、Fujiwara Keiichi、Kunitoh Hideo、Oba Mari S.、Wada Hideo、Hosokawa Jun、Takita Atsushi、Ikeda Masataka
    • Journal Title

      International Journal of Clinical Oncology

      Volume: 29 Pages: 407~416

    • DOI

      10.1007/s10147-024-02475-6

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biomarkers of Hypercoagulability in COVID-192023

    • Author(s)
      Wada Hideo
    • Journal Title

      Journal of Clinical Medicine

      Volume: 12 Pages: 3525~3525

    • DOI

      10.3390/jcm12103525

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diagnostic and treatment guidelines for thrombotic thrombocytopenic purpura (TTP) in Japan 20232023

    • Author(s)
      Matsumoto Masanori、Miyakawa Yoshitaka、Kokame Koichi、Ueda Yasunori、Wada Hideo、他
    • Journal Title

      International Journal of Hematology

      Volume: 118 Pages: 529~546

    • DOI

      10.1007/s12185-023-03657-0

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clot Waveform Analysis for Hemostatic Abnormalities2023

    • Author(s)
      Wada Hideo、Shiraki Katsuya、Matsumoto Takeshi、Shimpo Hideto、Shimaoka Motomu
    • Journal Title

      Annals of Laboratory Medicine

      Volume: 43 Pages: 531~538

    • DOI

      10.3343/alm.2023.43.6.531

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Thrombotic Mechanism Involving Platelet Activation, Hypercoagulability and Hypofibrinolysis in Coronavirus Disease 20192023

    • Author(s)
      Wada Hideo、Shiraki Katsuya、Shimpo Hideto、Shimaoka Motomu、Iba Toshiaki、Suzuki-Inoue Katsue
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 24 Pages: 7975~7975

    • DOI

      10.3390/ijms24097975

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Detection of Hypercoagulability in Patients with Acute Cerebral Infarction Using a Clot Waveform Analysis2023

    • Author(s)
      Kamon Toshitaka、Horie Shotaro、Inaba Tomoya、Ito Nobuo、Shiraki Katsuya、Ichikawa Yuhuko、Ezaki Minoru、Shimpo Hideto、Shimaoka Motomu、Nishigaki Akisato、Shindo Akihiro、Wada Hideo
    • Journal Title

      Clinical and Applied Thrombosis/Hemostasis

      Volume: 29 Pages: ー

    • DOI

      10.1177/10760296231161591

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Plasma Soluble Fibrin Is Useful for the Diagnosis of Thrombotic Diseases2023

    • Author(s)
      Ezaki Minoru、Wada Hideo、Ichikawa Yuhuko、Ikeda Nozomi、Shiraki Katsuya、Yamamoto Akitaka、Moritani Isao、Shimaoka Motomu、Shimpo Hideto
    • Journal Title

      Journal of Clinical Medicine

      Volume: 12 Pages: 2597~2597

    • DOI

      10.3390/jcm12072597

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Super Formula for Diagnosing Disseminated Intravascular Coagulation Using Soluble C-Type Lectin-like Receptor 22023

    • Author(s)
      Yamamoto Akitaka、Wada Hideo、Tomida Masaki、Ichikawa Yuhuko、Ezaki Minoru、Shiraki Katsuya、Shimaoka Motomu、Iba Toshiaki、Suzuki-Inoue Katsue、Kawamura Masahide、Shimpo Hideto
    • Journal Title

      Diagnostics

      Volume: 13 Pages: 2299~2299

    • DOI

      10.3390/diagnostics13132299

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] “Communication from the Scientific and Standardization Committee of the International Society on Thrombosis and Hemostasis on sepsis-induced coagulopathy in the management of sepsis”: Comment from Wada et al2023

    • Author(s)
      Wada Hideo、Matsumoto Takeshi、Shiraki Katsuya
    • Journal Title

      Journal of Thrombosis and Haemostasis

      Volume: 21 Pages: 1677~1678

    • DOI

      10.1016/j.jtha.2023.02.024

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Evaluation of Clot Waveform Analyses for Assessing Hypercoagulability in Patients Treated with Factor VIII Concentrate2023

    • Author(s)
      Matsumoto Takeshi、Wada Hideo、Shiraki Katsuya、Suzuki Kei、Yamashita Yoshiki、Tawara Isao、Shimpo Hideto、Shimaoka Motomu
    • Journal Title

      Journal of Clinical Medicine

      Volume: 12 Pages: 6320~6320

    • DOI

      10.3390/jcm12196320

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clinical Significance of Plasma Tenascin-C Levels in Recipients With Prolonged Jaundice After Living Donor Liver Transplantation2023

    • Author(s)
      Shinkai Toru、Kuriyama Naohisa、Usui Masanobu、Hayasaki Aoi、Fujii Takehiro、Iizawa Yusuke、Tanemura Akihiro、Murata Yasuhiro、Kishiwada Masashi、Katoh Daisuke、Matsumoto Takeshi、Wada Hideo、Yoshida Toshimichi、Isaji Shuji、Mizuno Shugo
    • Journal Title

      Transplantation Proceedings

      Volume: 55 Pages: 913~923

    • DOI

      10.1016/j.transproceed.2023.01.028

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sepsis-Associated DIC with Decreased Levels of Antithrombin and Fibrinogen is the Target for Combination Therapy with Thrombomodulin Alfa and Antithrombin2023

    • Author(s)
      Wada Hideo、Kawasugi Kazuo、Honda Goichi、Kawano Noriaki、Uchiyama Toshimasa、Madoiwa Seiji、Takezako Naoki、Suzuki Kei、Seki Yoshinobu、Ikezoe Takayuki、Iba Toshiaki、Okamoto Kohji
    • Journal Title

      TH Open

      Volume: 07 Pages: e65~e75

    • DOI

      10.1055/a-2009-9073

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Soluble C-type lectin-like receptor 2 in stroke (CLECSTRO) study: protocol of a multicentre, prospective cohort of a novel platelet activation marker in acute ischaemic stroke and transient ischaemic attack2023

    • Author(s)
      Uchiyama Shinichiro、Suzuki-Inoue Katsue、Wada Hideo、Okada Yasushi、Hirano Teruyuki、Nagao Takehiko、Kinouchi Hiroyuki、Itabashi Ryo、Hoshino Haruhiko、Oki Koichi、Honma Yutaka、Ito Nobuo、Sugimori Hiroshi、Kawamura Masahide
    • Journal Title

      BMJ Open

      Volume: 13 Pages: e073708~e073708

    • DOI

      10.1136/bmjopen-2023-073708

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The antithrombin activity recovery after substitution therapy is associated with improved 28-day mortality in patients with sepsis-associated disseminated intravascular coagulation2023

    • Author(s)
      Iba Toshiaki、Tanigawa Tomoki、Wada Hideo、Levy Jerrold H.
    • Journal Title

      Thrombosis Journal

      Volume: 21 Pages: ー

    • DOI

      10.1186/s12959-023-00556-6

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Application of a scoring system in Japanese patients diagnosed with atypical hemolytic uremic syndrome to assess the relationship between the score and clinical responses to eculizumab2023

    • Author(s)
      Wada Hideo、Teranishi Hirofumi、Shimono Akihiko、Kato Noritoshi、Maruyama Shoichi、Matsumoto Masanori
    • Journal Title

      Thrombosis Journal

      Volume: 21 Pages: ー

    • DOI

      10.1186/s12959-023-00489-0

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reply to Ishikura, H. What Does Soluble C-Type Lectin-like Receptor 2 (sCLEC-2) × D-Dimer/Platelet (PLT) (sCLEC-2 × D-Dimer/PLT) Mean for Coagulation/Fibrinolysis Conditions? Comment on “Yamamoto et al. Super Formula for Diagnosing Disseminated Intravascular Coagulation Using Soluble C-Type Lectin-like Receptor 2. Diagnostics 2023, 13, 2299”2023

    • Author(s)
      Wada Hideo、Yamamoto Akitaka、Shiraki Katsuya、Shimpo Hideto
    • Journal Title

      Diagnostics

      Volume: 14 Pages: 42~42

    • DOI

      10.3390/diagnostics14010042

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Anti Xa assays: current role in antithrombotic therapy.2023

    • Author(s)
      Hideo Wada
    • Organizer
      12th Congress of The Asian-Pacific Society of Thrombosis and Haemostasis (APSTH)
    • Int'l Joint Research / Invited
  • [Presentation] CWA による自己免疫性後天性凝固異常症の診断 CWA解析のUPDATE2023

    • Author(s)
      和田英夫
    • Organizer
      第17回日本血栓止血学会SSCシンポジウム
    • Invited
  • [Presentation] DIC診断・治療における臨床検査の役割2023

    • Author(s)
      和田英夫
    • Organizer
      第45回日本血栓止血学会学術集会
    • Invited
  • [Presentation] COVID-19の止血異常と血小板活性化2023

    • Author(s)
      和田英夫
    • Organizer
      第45回日本血栓止血学会学術集会
    • Invited

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Published: 2024-12-25  

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