2023 Fiscal Year Research-status Report
Cardiac electrophysiology evaluation system for improved drug assessment
| Project/Area Number |
22K12801
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| Research Institution | Osaka University |
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Principal Investigator |
李 俊君 大阪大学, 大学院工学研究科, 特任准教授(常勤) (10723786)
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| Co-Investigator(Kenkyū-buntansha) |
劉 莉 大阪大学, 大学院医学系研究科, 特任准教授 (50380093)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Keywords | pluripotent stem cells / cardiomyocytes / maturation / regenerative medicine |
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| Outline of Annual Research Achievements |
This year, we successfully integrated 2D traveling wave devices into 24-well plates for high-throughput assays. The traveling wave could be maintained stably for long periods. We also attempted to prepare a smaller device for 96-well plates. However, due to the shorter closed-loop circuit in the 96-well plate, we found that the traveling wave became unstable and disappeared after prolonged culture. Therefore, we utilized the 24-well plate device for subsequent electrophysiology assays on MEA system. iPSC-derived cardiomyocytes have been paced and matured. After a 14-day training period, the mature cardiac tissue was used for recording electrophysiological signals, which were clear and well-defined, indicating readiness for subsequent drug testing.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have accomplished most of the objectives of the second year rather smoothly. We are now working according to the third-year plan.
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| Strategy for Future Research Activity |
Next year, we will conduct multiple drug tests with varying risks of torsades de pointes (TdP) and compare the response of cardiac tissue at different stages of maturation. We will also validate the robustness and superiority of the traveling wave system in its application in drug assessment.
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