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2022 Fiscal Year Research-status Report

Active biological fluids

Research Project

Project/Area Number 22K14012
Research InstitutionThe University of Tokyo

Principal Investigator

Schnyder Simon  東京大学, 生産技術研究所, 特任助教 (50812616)

Project Period (FY) 2022-04-01 – 2025-03-31
Keywordssimulation / cell cycle / active matter / collective behavior
Outline of Annual Research Achievements

We have completed a first project in which the cell competition between cell types is studied in detail. For this we extended the model to include programmed cell death (apoptosis) and have it be regulated by the cell cycle. We were then able to study the invasion of one tissue into another as a function of the apoptosis and cell cycle parameters. In particular, we found the invasiveness of a tissue to be enhanced by the ability of cells to resist higher pressures and by lowered apoptotic rates. This was a necessary first step to move towards a better understanding of non-volume conserving fluids by studying cell tissue dynamics. Interestingly, we found that synchronization in the cell division/apoptosis events can emerge, leading to oscillations in pressure and cell-cycle activity.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

My study of non-volume conserving fluids is ongoing. In particular, I am currently in the process of implementing cell motility. I am in contact with two experimental groups who are providing me with data so as to be able to make comparisons with our model.

The results obtained from the first study are promising and have helped attract the attention of one of the two experimental collaborators, who are studying the non-volume conserving expansion of MDCK colonies, while employing new techniques to manipulate the cell’s activity and cell cycle. This makes our simulations an ideal match for their experiments. We are now in the process of using the experiments to calibrate the simulations.

The second experimental cooperation aims to understand more closely the collective dynamics of Dictyostelium discoideum. We are using combining experimental data, my simulations, and a novel machine learning approach.

Strategy for Future Research Activity

Presented with the opportunity to work with these two groups, I adjusted the goals of this project to maximize its impact.

I plan to complete the implementation of cell motility. With the experimental data of the two groups we hope to be able to make substantial progress towards the understanding of the interplay of cell mechanics and cell cycle regulation. With the MDCK system, we aim to understand how the colony expansion is affected by different properties of the cells such as the density, cell cycle activity and how they are distributed across the system. In the second study, we aim to infer the cellular interactions of Dictyostelium discoideum cells by studying how they self-organize.

Furthermore, we plan to be able to study the role of fluid flow in tissues by the end of this project. For this, we plan to implement a mesoscopic coarse grained approach that can account for the conservation of total fluid volume in the system, including the cells and the surrounding medium.

Causes of Carryover

Because of restrictions due to Covid-19 and my efficient spending I was unable to spend this year's budget. I plan to make good use of it next fiscal year.

  • Research Products

    (6 results)

All 2023 2022

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Journal Article] Competition between cell types under cell cycle regulation with apoptosis2022

    • Author(s)
      Li Jintao、Schnyder Simon K.、Turner Matthew S.、Yamamoto Ryoichi
    • Journal Title

      Physical Review Research

      Volume: 4 Pages: 033156

    • DOI

      10.1103/PhysRevResearch.4.033156

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Cell sorting in Dictyostelium discoideum: modeling and force inference2023

    • Author(s)
      Masahito Uwamichi, Simon K. Schnyder
    • Organizer
      6th Regional Conference of the “Grant-in-Aid for Scientific Research on Innovative Areas” Research Project “Information physics of living matters”
    • Invited
  • [Presentation] Competition between cell types under cell cycle regulation with apoptosis2023

    • Author(s)
      Jintao Li, Simon K. Schnyder, Matthew S. Turner, Ryoichi Yamamoto
    • Organizer
      Active Matter Workshop 2023
  • [Presentation] Cell competition in a hybrid mechanochemical model2022

    • Author(s)
      Jintao Li, Simon K. Schnyder, Matthew S. Turner, Ryoichi Yamamoto
    • Organizer
      Active days EUTOPIA Challenges in Active Matter
    • Int'l Joint Research / Invited
  • [Presentation] Cell competition in a hybrid mechanochemical model2022

    • Author(s)
      Jintao Li, Simon K. Schnyder, Matthew S. Turner, Ryoichi Yamamoto
    • Organizer
      25th Anniversary Symposium of German-Japanese Joint Research Project on Nonequilibrium Statistical Physics Perspectives for Future Collaboration
    • Int'l Joint Research / Invited
  • [Presentation] Role of the cell cycle in collective cell dynamics2022

    • Author(s)
      Jintao Li, Simon K. Schnyder, Matthew S. Turner, Ryoichi Yamamoto
    • Organizer
      The 74th Annual Meeting of the Japan Society for Cell Biology
    • Invited

URL: 

Published: 2023-12-25  

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