2023 Fiscal Year Final Research Report
Development and advanced utilization of unexplored culture environment for submerged culture with microorganisms via gas phase
| Project/Area Number |
22K14544
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | 微生物培養 / 液内振盪培養 / 振盪フラスコ培養 / ガス制御システム / 二酸化炭素換気能 / 酸素供給能 / 換気エンハンサー / モニタリングデバイス |
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| Outline of Final Research Achievements |
This study was challenged to "develop the unexplored culture environment via gas phase". In conventional shake-flasks, carbon dioxide (CO2) ventilation capacity was much smaller than oxygen (O2) supply capacity, induce the unintentional gas changes. The gas phase of shake-flasks during microbial cultivation used gaseous incubator were monitored. As a result, it was cleared that the flask gas phase was filled over gaseous CO2 more than the set point. The developed ventilation enhancer combined with the gas incubator including shaking table was able to be control the gas conditions according to the set point, while maintaining the components and convenience of the conventional shaking culture method. A plug system forced aeration for microbial culture of multiple shaken vessels suppresses volatilization, was developed.
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| Free Research Field |
生物工学
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| Academic Significance and Societal Importance of the Research Achievements |
フラスコなどの培養器を用いた液内振盪培養は、採取した環境試料中の有用微生物の集積、微生物を用いた生産技術の開発や改善を図るうえで、簡便に培養スクリーニングができる重要なバイオ技術である。原則、培養器の換気能の向上や強制的な通気をしない限り、ガス制御できないことが明らかとなった（非意図的なガス変動は、純粋培養と集積培養の両方に影響を及ぼす）。開発した換気エンハンサーとガスインキュベータの併用や、培地の揮発を抑制できる液内振盪培養用の通気プラグシステムを用いることで、培養器の気相環境を再現よく簡便に制御できる。バイオプロセス開発上流や培養を介した微生物および機能性成分の探索への導入が期待できる。
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