2022 Fiscal Year Research-status Report
Self-Powered Photoelectrochemical Device for Ultrasensitive Virus Detection using Multi-component Nanoheterostructure
| Project/Area Number |
22K14711
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
GANGANBOINA AkhileshBabu 国立研究開発法人物質・材料研究機構, 若手国際研究センター, ICYS研究員 (90937252)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | Virus sensor / Photoelectrochemical / self powered / Nanohetrostructure / Biosensor |
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| Outline of Annual Research Achievements |
Self-powered photoelectrochemical (PEC) sensors have recently attracted a lot of attention in healthcare systems. PEC detection inherits the benefits of both electrochemical and optical techniques as the photodriven electrochemical reaction is fundamental to PEC analysis, making it necessary to increase the associated PEC signals for sensitive detection. Investigations into the production, separation, and interfacial-redox-reaction mechanisms of photoinduced carriers are crucial for the realization of effective PEC detection at present. However, the slow interfacial interactions of the photogenerated carriers, as well as the necessity for suitable photoactive layers for sensing, continue to pose obstacles to the development of sophisticated PEC platforms. The important achievement in this work is well-defined novel architecture of Co3O4-CdSe-CdS-Pt using hollow sphere, rod and quantum dot allowing to adjust Pt and Co3O4 position at a particular distance regulates the hole and electron recombination rate, improving the photo-catalytic performance. The detection signal is majorly based on the photo current response generated by novel electrocatalyst Co3O4-CdSe-CdS-Pt upon excitation with visible light. For the amplification of the detection signal, Co3O4-CdSe-CdS-Pt will be encapsulated inside the liposome, functionalized with antibodies. Conjugating the Co3O4-CdSe-CdS-Pt encapsulated liposomes with virus, followed by magnetic separation allows the variation of analytical signal in response to virus concentration.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Synthesis of Co3O4-CdSe-CdS-Pt nanocomposite.
Sequential formation of nano heterostructure (Co3O4-CdSe-CdS-Pt) with increasing complexity comprised of four stages. The synthesis steps begin with forming cobalt oxide nanoparticles (Co3O4 NPs) by decomposition of cobalt nitrate with benzylamine in the presence of ammonium hydroxide. Then, the CdSe QD was grown on the surface of cobalt oxide nanoparticles. The cobalt oxide-CdSe complex synthesized serve as a seed for the CdS rod growth. In the dimer CdSe serve as nucleation site for the rod growth, and the rod grew exclusively on CdSe portion of the dimer leaving Cobalt oxide one end of the rod. Finally, the rods were tipped with Pt nanoparticle on another end of the rod.
To date, this is the first report synthesizing the nano heterostructure, Co3O4-CdSe-CdS-Pt for PEC based virus detection. We were also successful in encapsulation of the prepared Co3O4-CdSe-CdS-Pt NPs in to liposomes for the signal amplification
Currently, most of the efforts are focused on the synthesis, optimizing the growth conditions, understanding the chemical reactions, and improving reproducibility. Based on the gained knowledge on synthesis of Co3O4-CdSe-CdS-Pt, optimizing the distance between Co3O4 and Pt NPs which play an important role in interfacial-redox-reaction mechanisms increased substantially.
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| Strategy for Future Research Activity |
Synthesis of wafer-scale 2D TMDC single crystals.
Beside the successful synthesis of Co3O4-CdSe-CdS-Pt, it is also important to evaluate the quality and uniformity of the prepared nanoparticles. More characterization will be employed to make sure there is uniformity in particle size. More PEC electrodes will be fabricated and tested. With the photo current intensity and uniformity information, growth conditions will be further optimized.
Sensor preparation and its detection measurements:
The antibody-conjugated Co3O4-CdSe-CdS-Pt NPs -embedded liposome NVs and Ab-Fe3O4 will be mixed with different concentration of viruses, allowing formation of sandwich-structured immunocomplex. The exact ratio of each precursor with the optimum condition to get the best suited immunocomplexes can be optimized carefully. The formed immunocomplex will be isolated from the sample matrix by simple magnetic separation. Using surfactants, the separated liposome NVs can be melted and release several Co3O4-CdSe-CdS-Pt NPs. The released Co3O4-CdSe-CdS-Pt NPs act as photocatalyst and are deposited on to electrode. Thus, prepared electrode is placed in to the electrolyte chamber and measure the photo current.
After the optimization of synthesis and detection prameters, we will also perform the detection of virus in real samples.
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| Causes of Carryover |
The left funding is due to the delay in ordering the antigen and antibodies as the optimizng the sensing parameters is yet to be completed. The remaining funding will be used for ordering the required consumables in the next fiscal year
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