2022 Fiscal Year Research-status Report
Control morphology and drug release of nanodrugs through templated crystallization of poorly water-soluble compounds
| Project/Area Number |
22K14742
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2026-03-31
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| Keywords | nanodrugs / metallic cluster / reprecipitation / crystallization / cancer therapy |
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| Outline of Annual Research Achievements |
The purpose of this research is to design and fabricate a carrier-free nanodrug via templated-crystallization method using SN-38, the active metabolite of irinotecan, acts as a potent inhibitor of DNA topoisomerase I. In this work, several derivatives of SN-38 were selected as the anticancer moiety, and Au cluster of ca. 10 nm was selected as a template. The nanodrugs are fabricated by reprecipitation method. The modified Au/SN38-Chol nanoparticles were successfully fabricated in several sizes by adjusting the reprecipitation conditions. The existence of Au in the nanodrugs was confirmed in the UV-Vis spectra with visible absorbance at ca. 550 nm. The formation yield of the hybrid nanodrugs were suggested to be affected by the hydrophilicity of Au surface.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The first target of the research plan, including establishing the fabrication method for each component of the hybrid nanodrugs and the combination into a single nanoparticle, are close to complete. The research is progressing to the optimization of the nanodrug’s morphologies and compositions, and the correlation between that and drug release is carried out in parallel.
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| Strategy for Future Research Activity |
The next targets of the research are investigation of drug release under photothermal effect of the obtained nanodrugs, and their in vitro cytotoxicity.
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| Causes of Carryover |
Due to changing of research location, several instruments that are required in the later part of the research plan became unavailable at the new location. Therefore, a part of the budget was transferred in order to obtain new instruments.
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