2022 Fiscal Year Research-status Report
Development of novel Immunosynbiotics with wakame utilizing immunobiotics and immunogenics
| Project/Area Number |
22K14982
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| Research Institution | Tohoku University |
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Principal Investigator |
Binghui Zhou 東北大学, 農学研究科, 助教 (70906031)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | Immunobiotics / Immunosynbiotics / Wakame / Lactobacilli / Porcine intestinal cell / Tolerance to acid |
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| Outline of Annual Research Achievements |
Antibiotic resistance caused by the overuse of antibiotics in livestock production has been a severe problem, leading to the need for effective alternatives. A promising alternative to antibiotics is the "immunosynbiotics," a combination of immunobiotics and immunoprebiotics. The seaweed wakame is considered an immunoprebiotic, and L. salivarius is an immunobiotic, isolated from the intestine of wakame-fed pigs. Adding wakame to pig feed has been found to improve the immune response and alter the microbiota in the porcine large intestine. Using wakame as a feed supplement could also provide an economic recovery alternative in Miyagi prefecture after the 2011 Tohoku earthquake. This year, three results were achieved in this study:
(1)Eight lactobacilli strains selected in previous studies were able to grow better in wakame-based broths with the addition of B vitamins. The comparative genomic analysis revealed that the differences between the strains were related to their different wakame utilizing abilities.
(2)One strain that had adhesion ability to porcine intestinal epithelial cells could survive under the simulated gastric juice in the presence of wakame because the fiber in wakame could protect the cells from the severe environment of acid.
(3)The growth and storage conditions have various effects on the characteristics of lactic acid bacteria, especially high-temperature and low-temperature stress The incubation condition of the selected strain was determined. In future research, the strain under determined incubation conditions will be provided for the in vivo study.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
In this study, several significant findings were obtained that can potentially aid in the development of Immunosynbiotics as effective alternatives to antibiotics in the pig industry.
(1) The research showed that eight lactobacilli strains were able to grow better in wakame-based broths developed in the study, and comparative genomic analysis revealed the differences between the strains were related to their different abilities to utilize enzyme-treated wakame. Additionally, one immunobiotic strain demonstrated the ability to survive under simulated gastric juice in the presence of wakame due to the protective effect of the fiber contained in wakame.
(2) The study found that growth and storage conditions have various effects on the characteristics of lactic acid bacteria, which can affect their adhesion to host cells and immune-regulating properties.
(3) The research determined the incubation conditions of the selected lactobacilli strain for future in vivo studies.
Overall, the findings of this study provide solid data supporting the use of wakame-based immunosynbiotics in pig production and can aid in the development of effective alternatives to antibiotics, potentially reducing the problem of antibiotic resistance in intensive livestock production
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| Strategy for Future Research Activity |
Based on the progress of the research on immunosynbiotics for pig feed, the future in vivo study will involve feeding the porcine subjects with the selected lactobacilli strains grown in wakame-based broths containing enzyme-treated wakame leaf or wakame stalk with the addition of B vitamins. The study will investigate the effects of the immunosynbiotic combination on the pig's immune response, gut microbiota, and growth performance compared to pigs fed with traditional antibiotic-based feed. The results of this study could provide valuable insights into the effectiveness of immunosynbiotics as an alternative to antibiotics in the pig industry and could contribute to the development of sustainable and eco-friendly livestock production.
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| Causes of Carryover |
The reason for Incurring Amount to be used next fiscal year is the Delayed purchase of research equipment. 600,000 Yen to be used next year to purchase the shaking OD equipment and necessary supplies. The new equipment will enhance research capabilities, replace outdated machinery, and enable advanced experiments. The remaining funds will be used to purchase research supplies and materials for accurate and precise experiments. Funds may also be allocated towards attending research conferences or events.
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Research Products
(2 results)
