Elucidation of mechanisms that control the fidelity of homologous recombination using in vitro analyses

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K15036
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: Kyushu University
• Principal Investigator: Kawasoe Yoshitaka 九州大学, 理学研究院, 助教 (60805422)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: DNA修復 / 相同組換え / ミスマッチ修復 / DNA二重鎖切断損傷 / 試験管内再構成 / ツメガエル卵抽出液

Outline of Final Research Achievements

Homologous recombination (HR) is one of the important pathways to repair DNA double-strand breaks (DSBs). It utilizes the sequence homology around DSB sites to repair DSBs. In this study, I aimed to elucidate the regulatory mechanisms that control the fidelity of HR by reconstituting the reaction using purified proteins. Up to now, I have purified all the factors predicted to be required for the reconstitution. They have almost the same activities as the endogenous proteins in our biochemical experimental system. I divided the reaction required to control the fidelity of HR into three reactions and reconstituted the key reaction in each reaction.

Free Research Field

DNA修復・複製

Academic Significance and Societal Importance of the Research Achievements

ゲノム中にはよく似た配列が多数存在し、それらは誤った組換えの原因となる。相同組換えの正確性の破綻は、染色体異常や染色体喪失などを引き起こし、それらは細胞のがん化や細胞死につながる。一方で、遺伝的な多様性を生み出すためには、ある程度の配列不一致を許容して組換えを行う必要がある。本研究によって、組換えの正確性を制御する反応が理解されれば、基礎科学だけでなく医学的な側面などへも、重要な知見を提供できることが期待される。