2023 Fiscal Year Annual Research Report
Study of enzymes inside liquid-liquid phase separated crowded droplets
| Project/Area Number |
22K15065
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
DINDO Mirco 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 客員研究員 (10933088)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | Phase separation / Chemotaxis / TCA cycle / Enzymes / Catalysis |
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| Outline of Annual Research Achievements |
During the current project we developed synthetic droplets prepared using phase separation which are able to freely move following a chemical gradient (published in JACS). Also, by using the same liquid phase separated system we studied the interaction between two non consecutive enzymes involved in the TCA cycle (just accepted, Nat Comm) within the droplets. Another research article is preparation where we studied the effects of the phase separation on the catalytic activity of two enzymes. In this study we have also analyzed the effects of the enzymatic activity on the material properties of the liquid phase separated droplets.
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