2023 Fiscal Year Final Research Report
Adaptive RNA editing in Cephalopods
| Project/Area Number |
22K15085
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43050:Genome biology-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | RNA editing / cephalopods / single-cell mRNA / neural organs |
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| Outline of Final Research Achievements |
I successfully standardized a protocol for the isolation and sorting of high-quality single cells from the optic lobe of squid. I have also identified distinct cellular populations in squids that are 1 to 7 days old, differing in size and granularity. Sequencing the mRNA of these high-quality cells revealed isoform-specific editing in dopaminergic gene markers such as Ty3h, particularly in the UTR regions. Additionally, we found that RNA editing occurs significantly in transposable elements without a clear pattern of cell-type specificity.
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| Free Research Field |
Evolutionary Biology
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| Academic Significance and Societal Importance of the Research Achievements |
We found that dopaminergic cell types, the most abundant in the optic lobe, exhibit differential RNA-editing patterns in gene markers, particularly in regulatory regions. We expect to fully characterize cephalopod-specific and evolutionarily conserved regulatory networks that underlie RNA editing.
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