2023 Fiscal Year Final Research Report
Adaptive RNA editing in Cephalopods
Project/Area Number |
22K15085
|
Research Category |
Grant-in-Aid for Early-Career Scientists
|
Allocation Type | Multi-year Fund |
Review Section |
Basic Section 43050:Genome biology-related
|
Research Institution | Okinawa Institute of Science and Technology Graduate University |
Principal Investigator |
|
Project Period (FY) |
2022-04-01 – 2024-03-31
|
Keywords | RNA editing / cephalopods / single-cell mRNA / neural organs |
Outline of Final Research Achievements |
I successfully standardized a protocol for the isolation and sorting of high-quality single cells from the optic lobe of squid. I have also identified distinct cellular populations in squids that are 1 to 7 days old, differing in size and granularity. Sequencing the mRNA of these high-quality cells revealed isoform-specific editing in dopaminergic gene markers such as Ty3h, particularly in the UTR regions. Additionally, we found that RNA editing occurs significantly in transposable elements without a clear pattern of cell-type specificity.
|
Free Research Field |
Evolutionary Biology
|
Academic Significance and Societal Importance of the Research Achievements |
We found that dopaminergic cell types, the most abundant in the optic lobe, exhibit differential RNA-editing patterns in gene markers, particularly in regulatory regions. We expect to fully characterize cephalopod-specific and evolutionarily conserved regulatory networks that underlie RNA editing.
|