Molecular analysis of novel wound-induced regulators of shoot regeneration in Arabidopsis

2022 Fiscal Year Annual Research Report

• Project/Area Number: 22K15150
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Coleman Duncan 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (10895311)
• Project Period (FY): 2022-04-01 – 2023-03-31
• Keywords: Regeneration / Arabidopsis / HSF / Plant

Outline of Annual Research Achievements

In FY 2022 My main contribution to this project is characterizing how the wound-activated HEAT SHOCK FACTOR A1 (HSFA1) promotes shoot regeneration in Arabidopsis. I utilized a combination of genetic, biochemical and bioinformatic tools to reveal a novel mechanism of wounding-induced shoot regeneration.
Key research findings: 1) Determined key downstream targets of HSFA1 using a bioinformatics approach. 2) Verified HSFA1 binding to direct targets using biochemistry. 3) Generated and phenotyped genetic crosses to confirm epistasis of genes involved in wound-induced cellular reprograming. 4) Observed the nuclear translocation of HSFA1d in response to wounding using live -The results have been compiled into a manuscript for submission in 2023
Collaborations (informal)
1) Ari Sadanandom (Durham University, UK) – Shared plant material and jointly wrote a grant proposal to Biotechnology and Biological Sciences Research Council (BBSRC) to investigate SUMOylation in cellular reprograming. 2) Teruaki Taji (Tokyo University of Agriculture) – Provided cDNA sample for qPCR analysis to investigate the role of HSFA1 in photomorphogenesis. 3) Yutaka Kodama (Utsunomiya University) – Visited laboratory and learned techniques for imaging leave epidermal cells with a confocal microscope.
Conferences: 1)Oral - Annual conference for Society of Experimental Biology, Montpellier France.2) Oral - Satellite meeting on SUMO modification in cell signalling – Society of Experimental Biology, Montpellier France – Invited speaker. 3)Poster - CSRS Annual progress meeting 2022

Research Products

• [Journal Article] Molecular Mechanisms of Plant Regeneration from Differentiated Cells: Approaches from Historical Tissue Culture Systems (2022)
  • Author(s): Morinaka Hatsune、Coleman Duncan、Sugimoto Keiko、Iwase Akira
  • Journal Title: Plant and Cell Physiology Volume: 64 Pages: 297～304
  • DOI: 10.1093/pcp/pcac172
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] The role of SUMOylation during shoot regeneration in Arabidopsis (2022)
  • Author(s): Duncan Coleman
  • Organizer: Annual conference for Society of Experimental Biology, Montpellier France
  • Int'l Joint Research
• [Presentation] The role of SUMOylation during shoot regeneration in Arabidopsis (2022)
  • Author(s): Duncan Coleman
  • Organizer: Satellite meeting on SUMO modification in cell signalling & Society of Experimental Biology, Montpellier France
  • Int'l Joint Research / Invited