2023 Fiscal Year Annual Research Report
Neural mechanisms underlying behavioral flexibility
| Project/Area Number |
22K15633
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
サポン ギデオン 沖縄科学技術大学院大学, 神経生物学研究ユニット, ポストドクトラルスカラー (00837198)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | reversal learning / acetylcholine / striatum |
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| Outline of Annual Research Achievements |
Multiple lines of evidence documented from functional neuroimaging, pharmacological and lesion studies, and computational modeling illustrate the involvement of striatal acetylcholine (ACh) in forms of flexible behaviours such as reversal learning and set-shifting. Despite this emerging framework, there has not been any direct recording of the spatiotemporal dynamics of ACh in behavioural tasks requiring flexible responding. To address this, I designed a closed-loop virtual-reality response learning task for head-fixed mice using the jetball system. To characterize ACh activity, I have commenced in vivo imaging using 2-photon microscopy via a GRIN lens implanted above the striatum and a genetically encoded biosensor that permits ultrafast cellular resolution imaging of ACh, iAChSnFR.
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