2023 Fiscal Year Research-status Report
The regulatory mechanism of sympathetic nerves on eosinophil-associated micr o-inflammation in animals with IBS
| Project/Area Number |
22K15656
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
段 ショウキ 兵庫医科大学, 医学部, 助教 (20910607)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Keywords | SNS / IBS / Micro-inflammation / Visceral pain |
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| Outline of Annual Research Achievements |
This year, I primarily conducted genetic modulation experiments. Using DBH-cre mice and AAV-PHP.s-Gq, I successfully activated sympathetic nervous system (SNS) activity through CNO injection, as confirmed by double immunohistochemical staining of cre and mcherry, and the NE concentration in the serum. The results indicated that immune cells were recruited into the GI tract following SNS activation, consistent with findings in the MS model. As for the underlying mechanisms, I checked several cytokines and chemokines, and aims to clarify the connection between SNS activity and immune alteration.
I attended two academic conferences this year. I did symposium speaking in the 46th Annual Meeting of the Japan Neuroscience Society. And I did an oral presentation in the 45th Annual Meeting of Japanese Association for the Study of Pain.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I proved that activate SNS recruited immune cells into the GI tract using genetic modulation method. By those data, I achieved the second step of the project.
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| Strategy for Future Research Activity |
In the next year, I will clarify the underlying mechanism that SNS modulates the immune cell immigration. Especially the cytokines, chemokines and related cell type. In addition, I will summarize the data and prepare the manuscript to publish the article.
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| Causes of Carryover |
I am planning to attend an international conference next year, so I have allocated some budget for it. Regarding the experiment, the funds will be utilized for the procurement of animals, ELISA kits, and consumables. I will summarize the data in preparation for publication of the article. Additionally, the funds will cover the publishing fees.
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Research Products
(2 results)
