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2023 Fiscal Year Research-status Report

新規アッセイ法による肺炎球菌ワクチン接種幼児の唾液と血清における抗体レベルの比較

Research Project

Project/Area Number 22K15924
Research InstitutionNagasaki University

Principal Investigator

ドバデル ビム  長崎大学, 熱帯医学研究所, 准教授 (80761113)

Project Period (FY) 2022-04-01 – 2025-03-31
KeywordsMultiplex immunoassay / Pneumococcal vaccines / Antibody titer / children / vaccine immunogenicity
Outline of Annual Research Achievements

We have completed the validation of the microsphere-based assay for all 24 pneumococcal vaccine serotypes. WHO standard ELISA has been established following the protocol developed by the WHO.

We have got the ethical approval from the Institutional Review Board (IRB) of School of Tropical Medicine and Global Health. We have applied for ethical approval from Nepal Health Research Council. Our application has already reviewed and we have addressed the comments raised by the reviewers. Material transfer agreement between the Institute of Tropical Medicine and the Siddhi Memorial Hospital has been completed.

For data and sample collection from the research site, RedCap training is completed and other lab equipment, including a deep freezer of -80 degree centrigrate is established.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

The project is progressing smoothly although it has taken time to complete the laboratory part of assay development and validation along with establishment of WHO ELISA for quantitative assessment of antibodies against pneumococcal vaccine serotypes.

Strategy for Future Research Activity

We expect to get ethical approval from Nepal Health Research Council by the end of this month. From next month, we plan to enroll children in this project along with collection of blood and saliva. We expected to finished data and sample collection of 300 children within 3 months of start of the project.

We will bring these serum and saliva samples to Institute of Tropical Medicine by the end of August and start testing them with the 24-plex assay. We plan to get the results by October, and start preparing the report.

  • Research Products

    (4 results)

All 2024 2023

All Journal Article (3 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] A description of a pre-emptive typhoid Vi capsular polysaccharide vaccination campaign after the 2015 earthquake in Nepal and vaccine effectiveness evaluation2024

    • Author(s)
      Dhoubhadel Bhim Gopal、Sawada Ikumi、Shrestha Dhruba、Fukuya Yoshifumi、Raya Ganendra Bhakta、Nebie Eric Ipyn、Hayashi Yumiko、Pasakhala Rasila、Suzuki Motoi、Morimoto Konosuke、Parry Christopher M.、Ariyoshi Koya
    • Journal Title

      Tropical Medicine and Health

      Volume: 52 Pages: 1-8

    • DOI

      10.1186/s41182-024-00580-w

  • [Journal Article] The overlap of accessory virulence factors and multidrug resistance among clinical and surveillance Klebsiella pneumoniae isolates from a neonatal intensive care unit in Nepal: a single-centre experience in a resource-limited setting2024

    • Author(s)
      Shrestha Raj Kumar、Shrestha Dhruba、Kunwar Ajaya Jang、Thapa Sandeep、Shrestha Nipun、Dhoubhadel Bhim Gopal、Parry Christopher M.
    • Journal Title

      Tropical Medicine and Health

      Volume: 52 Pages: 1-5

    • DOI

      10.1186/s41182-024-00595-3

  • [Journal Article] A major dengue epidemic in 2022 in Nepal: need of an efficient early-warning system2023

    • Author(s)
      Dhoubhadel Bhim Gopal、Hayashi Yumiko、Domai Fleurette Mbuyakala、Bhattarai Suraj、Ariyoshi Koya、Pandey Basu Dev
    • Journal Title

      Frontiers in Tropical Diseases

      Volume: 4 Pages: 01-05

    • DOI

      10.3389/fitd.2023.1217939

  • [Presentation] Development of a Novel 24-Plex Quantitative Microsphere-Based Assay for the Assessment of Antibodies Against Pneumococcal Vaccines2024

    • Author(s)
      Yumiko Hayashi, Kentaro Kato, Konosuke Morimoto, Koya Ariyoshi, Bhim Gopal Dhoubhadel
    • Organizer
      13th Meeting of the International Society of Pneumonia and Pneumococcal Diseases (ISPPD-13)
    • Int'l Joint Research

URL: 

Published: 2024-12-25  

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