2023 Fiscal Year Final Research Report
Rapid identification of black fungi by MALDI-TOF MS (Mass Spectrometer)
| Project/Area Number |
22K16271
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53050:Dermatology-related
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | MALDI-TOF MS / 黒色真菌 / Exophiala |
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| Outline of Final Research Achievements |
Chromoblastomycosis is a rare but deadly problem worldwide. Treatment methods for chromoblastomycosis vary depending on the fungal species, and rapid identification is important. However, the problem is that it is highly specialized and time-consuming. This study uses MALDI-TOF MS to establish rapid identification of the genus Exophiala, which is a frequently occurring chromoblastomycosis. We created an in-house library by registering standard strains in currently available libraries. As a preparation method for testing, colonies created on slanted media were directly used.
With this preparation method, it was suggested that identification of the genus Exophiala is possible by enriching the MALDI-TOF MS library and devising the preparation method.
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| Free Research Field |
真菌
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| Academic Significance and Societal Importance of the Research Achievements |
黒色真菌症は稀であるが、皮膚のみでなく全身に播種し致死的であることから発展途上国を中心に世界中で問題となっている。黒色真菌症は菌種により治療法が異なり迅速に菌種を同定すること重要であるが、菌種同定は専門性が高く、時間を要する点が問題であった。MALDI-TOF MSのライブラリの充実と前処理法の工夫により、Exophiala属については同定が可能であると示唆された。また、今回確立した前処理法の様に、前処理法の工夫とライブラリの充実により、黒色真菌の迅速同定が可能であることが示唆された。
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