Pathological roles of immune guidance molecules that induce granulocytic cell death

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K16361
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54020:Connective tissue disease and allergy-related
• Research Institution: Osaka University
• Principal Investigator: Nishide Masayuki 大阪大学, 大学院医学系研究科, 助教 (80812255)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: 好中球 / 好酸球 / 細胞外トラップ / シングルセル解析

Outline of Final Research Achievements

A group of immune guidance molecules have distinct effects on extracellular trap formation and contribute to neutrophil- and eosinophil-associated diseases. We reported that integrin-mediated adhesion contributes to extracellular trap formation in vasculitis (Immunohorizons 2022) and that mitochondrial DNA contributes to neutrophil activation in Behcet's disease (EMBO J 2023). We also proposed a prognostic prediction based on immunological profiles of peripheral blood mononuclear cells from patients with vasculitis by subjecting them to single-cell analysis (Nat Commun 2023).

Free Research Field

リウマチ・膠原病内科学

Academic Significance and Societal Importance of the Research Achievements

本邦において厚生労働省指定難病に該当する顕微鏡的多発血管炎やベーチェット病について好中球活性化のメカニズムの一端を明らかにし、新たな治療戦略につながることが期待される。また、シングルセル解析を介して、好中球のみならず単球の遺伝子発現プロファイルに基づいた層別化に成功し、疾患予後予測へつながる成果を見いだした。