2023 Fiscal Year Final Research Report
The role of glycolysis in odontoblast differentiation and angiogenesis during reparative dentin formation
| Project/Area Number |
22K17009
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57010:Oral biological science-related
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | 象牙芽細胞 / 解糖系 / 血管新生 |
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| Outline of Final Research Achievements |
The importance of glycolysis in the differentiation of odontoblasts, which are responsible for reparative dentin formation, is unclear. In this study, we applied stimulation to mouse teeth and analyzed the role of glycolysis in the formation of reparative dentin induced by this stimulation. As a result, we confirmed that glycolytic enzymes were highly expressed in odontoblasts, which form reparative dentin after trauma, and that angiogenesis was also increased at the same time. We also confirmed that when reparative dentin formation was induced by administering a glycolytic enzyme inhibitor, the formation of reparative dentin was suppressed with a decrease in odontoblasts. These results suggest that glycolysis and the subsequent energy metabolic pathway play an important role in reparative dentin formation.
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| Free Research Field |
口腔組織
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| Academic Significance and Societal Importance of the Research Achievements |
修復象牙質形成時の象牙芽細胞分化において解糖系が関与するかは未解明の状態であった。実際の臨床に則した損傷モデルを用いて修復象牙質形成の誘導実験をおこなったところ、修復象牙質形成における解糖系の重要性が示唆された。そのため、象牙芽細胞における解糖系は治療標的として歯の治療に応用できる可能性がある。具体的には、歯の侵襲時に解糖系を効率的に上昇させることで修復象牙質を誘導し歯を保存させることができ、歯の再生においては、解糖系亢進により象牙芽細胞分化を促し、象牙質形成を効率的に行うことができる。本研究において、象牙芽細胞における解糖系は治療標的であり、歯の保存・再生療法に応用できる可能性が示唆された。
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