Development of minimally invasive and highly efficient alveolar bone regeneration therapy using a combination of new scaffold materials and human cultured periosteal cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K17072
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Niigata University
• Principal Investigator: Tsuzuno Takahiro 新潟大学, 医歯学総合病院, 特任助教 (40907383)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: ヒト培養骨膜細胞 / 培養骨膜細胞シート / 人工足場材 / 骨再生能

Outline of Final Research Achievements

Alveolar bone loss due to periodontitis adversely influences on oral health-related quality of life. Cell-based alveolar bone regeneration therapy using human cultured periosteal cells in combination with autologous bone exhibits great potential in the treatment of alveolar bone defects, but it is necessary to be combinated with new artificial scaffold materials for less invasive and highly efficient treatment. In this study, carbonate apatite granules and recombinant human collagen peptides were mixed with human cultured periosteal cells respectively and transplanted into calvarial bone defect model in vivo. From histological and micro-CT analysis, transplantation of these new scaffold materials with human cultured periosteal cells had great effect on bone regeneration compared to transplantation of these materials alone.

Free Research Field

歯周病学

Academic Significance and Societal Importance of the Research Achievements

本研究課題から、ヒト培養骨膜細胞と新規人工足場材である炭酸アパタイトおよびヒトⅠ型コラーゲン様リコンビナントペプチドとの併用移植によって優れた骨再生が認められた。実臨床の現行プロトコルにおいて、ヒト培養骨膜細胞との併用移植に用いられている自家骨採取は外科的侵襲が大きく、また採取骨量に制限がある。今後、ヒト骨培養骨膜細胞を用いた歯槽骨再生療法において、自家骨併用移植に代替可能な新規人工足場材の応用が期待される。