2022 Fiscal Year Research-status Report
Treatment of Periodontitis using stem cell like epithelial cell rests of Malassez
| Project/Area Number |
22K17259
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | Amleogenin / Clone / Malassez |
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| Outline of Annual Research Achievements |
Epithelial cell rests of Malassez (ERM) are an easy source of stem cell-like cells. Our previous work has successfully demonstrated that an ERM variant (isolated clone of ERM) can maintain healthy periodontal space by inhibiting ankylosis between the tooth and alveolar bone.
This study aims to use the applicant's previously isolated clone model to repair the lost or injured periodontal ligament (PDL) tissue to restore tooth form and function.
Phase-1: We obtained primary ERM from PDL tissues of porcine and isolated clones of ERM. We isolated clones 1-10. The characterization was done using immunofluorescence staining of ERM cell markers.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have already isolated clones and characterized their origin as Epithelial cell rests of malassez markers.
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| Strategy for Future Research Activity |
Phase-2: ERM clones will be checked for enamel matrix proteins and some stem cell markers.
Pilot experiments to combine some cells to produce cell sheets.
Phase-3: Histological study of the transplanted tooth. Next-generation sequencing of ERM clones with stem cell-like properties and analysis to identify essential genes.
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| Causes of Carryover |
Delays in delivery and price increases for required research reagents resulted in insufficient funds remaining to be used in the next fiscal year.
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