2023 Fiscal Year Annual Research Report
Establishing a low-cost next-generation sequencing platform for on-site real-time surveillance of SARS-CoV-2 and other viruses in an unreached community from the Democratic Republic of Congo
| Project/Area Number |
22K17352
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| Research Institution | Osaka Metropolitan University |
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Principal Investigator |
チバング・カバンバ エバリステ 大阪公立大学, 大学院医学研究科, 特任助教 (50900693)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | Viral genome / Nanopore sequecing / Congo / Hepatitis B virus / Monkeypox virus |
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| Outline of Annual Research Achievements |
In FY 2023, studies were conducted in Africa applying our developed toolkit. With the decline of the SARS-CoV-2 pandemic, our interest shifted towards investigating additional endemic viruses, including hepatitis B virus, Monkeypox virus, and other viruses potentially transmitted from mother to child. Overall, 1077 individuals, comprising 200 pregnant women and 200 newborns, participated in surveys conducted in rural populations of the DRC. However, challenges arose in deploying our virus sequencing toolkit directly in the field, primarily due to the complexities of safely delivering sequencing kits in specific storage conditions. Therefore, we decided to collect and transport samples to Japan for analysis using the toolkit, albeit resulting in prolonged wait times for sample transfer.
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