Elucidation of novel function of MRE11

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K18030
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 63020:Radiation influence-related
• Research Institution: Hiroshima University
• Principal Investigator: Shimizu Naoto 広島大学, 統合生命科学研究科(理), 助教 (10911589)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: MRE11 / 相同組換え / DNA修復

Outline of Final Research Achievements

I find that the MRE11 nuclease promotes homologous recombination not only in DNA double-strand break resection but also in post-resection in human TK6 cells. To investigate the mechanism of homologous recombination, I performed immune-staining and chromosome analysis using MRE11-null (MRE11-/-) mutant cells and nuclease-deficient (MRE11-/H129N) cells derived from the human TK6 cell line. The results show the nuclease activity of MRE11 promote the resolution of homologous recombination intermediate.

Free Research Field

DNA損傷修復

Academic Significance and Societal Importance of the Research Achievements

本研究によって、MRE11によるJM構造の解消を一分子レべルで可視化することができれば、JM構造解消機構を詳細に調べられる。JM構造解消機構を明らかにすることは、がんや一部の遺伝病の発症機構の解明に貢献する。