2023 Fiscal Year Final Research Report
Characterization and Quality Evaluation of Oral Mucosal Epithelial Cell Sheets for Corneal Disease Treatment
| Project/Area Number |
22K18168
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 90110:Biomedical engineering-related
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2022-04-01 – 2024-03-31
|
| Keywords | 再生医療 / 自家培養口腔粘膜上皮細胞シート / 角膜移植 / 両側性角膜上皮幹細胞疲弊症 / メカノバイオロジー / バイオインフォマティクス / 発現変動遺伝子 / マイクロアレイ解析 |
|---|
| Outline of Final Research Achievements |
This study aimed to examine the effects of substrates stiffness when manufacturing autologous cultured oral mucosa epithelial cell sheet (COMECS) on corneal wound healing and prognosis after COMECS transplantation by studying differential gene expressions. COMECSs were manufactured on three different substrates such as a thermo-responsive tissue culture dish (UpCell), a type I collagen fibril membrane (Cell Campus), and type I collagen hydrogel (Cellmatrix), whose Young’s modulus is 3 GPa, 1.76 MPa, and 5 kPa, respectively. Based on their microarray data, we performed bioinformatics analyses. The fold change ratios and the statistical differences of differential expressed genes (DEGs) between UpCell and Cellmatrix were not as large as those of UpCell and Cell Campu. Additionally, this study found that some DEGs were clearly present when COMECS was harvested from UpCell. Further analyses are required to assess how COMECSs affect wound healing of cornea when mfg different substrates.
|
| Free Research Field |
医歯薬学
|
| Academic Significance and Societal Importance of the Research Achievements |
COMECSは、硬いプラスチック製UpCell上で作製されるが、移植床となる眼球はUpCellよりはるかに軟らかい。作製過程のCOMECSの “品質/特性”は、移植後の環境変化に影響を受けることが予想される。本課題では足場材の硬さの違いがCOMECSの品質/特性にどのような影響を与えるか遺伝子レベルで検討した。本研究の継続により、COMECSの品質管理と移植予後向上に役立つ基盤形成を行い、COMECS治療の存在価値に貢献する。
|
