2023 Fiscal Year Research-status Report
Cell morphogenesis by the subcellular spatial segregation of molecular mobility on biomaterial surface
| Project/Area Number |
22K18200
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Han Aifang 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (30938832)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Keywords | micro-casting / cell morphogenesis / extracellular matrix |
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| Outline of Annual Research Achievements |
Micro-casting fabrication of multi collagen strips patterned surface has been
achieved. The addition of DMS-co-EO into Polydimethylsiloxane(PDMS) mold has improved the harvesting of collagen strips greatly. The underlying reason is due to the increased wettability of DMS-co-EO added PDMS mold compared to PDMS mold. The fibroblast cell elongation morphology has been observed onto the micro-patterned collagen strip surface.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
I take maternity leave and childcare leave during 2023.07-2024.03
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| Strategy for Future Research Activity |
I will continue to do other cell types (stem cell, muscle cell) to observe the cell morphology on the micropatterned extracellular matrix surface. Also, I will try different types of extracellular matrix (alumina, fibronectin) micropatterned on the device to detect the cell morphology.
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| Causes of Carryover |
1. I plan to fabricate micropatterns of different extracellular matrix to the micro-casting device system. Therefore, I need to buy some other extracellular matrix of alumina, fibronectin, etc. 2. I need to make more microdevices for the experiment. 3. Also, I plan to observe the migration of the cell on the micropatterned ECM, so I will buy a microscope that can be used in the incubator for movie recoding. 4. I will perform PCR experiments for analyzing the mechanisms
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