2023 Fiscal Year Final Research Report
Molecular dynamics of dehydration / freeze resistance
| Project/Area Number |
22K18694
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 13:Condensed matter physics and related fields
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Project Period (FY) |
2022-06-30 – 2024-03-31
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| Keywords | クリプトビオシス / ガラス転移 / トレハロース / 時間分解分光 / red-edge効果 / ２次元蛍光励起スペクトル / 耐乾燥性 / 耐凍結性 |
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| Outline of Final Research Achievements |
In order to elucidate the suppression of molecular motion by the saccharide glass transition, we conducted experiments utilizing fluorescence red-edge effect (REE) and femtosecond transient absorption (TA) spectroscopy. We measured the REE of Auramine O (AuO) in saccharide glasses and observed the suppression of torsional motion of the phenyl groups. When femtosecond TA spectroscopy was performed on a similar non-fluorescent dye, phenol blue (PhB), it was found that non-radiative decay was not suppressed even when it was encapsulated in polymer glass, suggesting that the non-radiative decay of PhB does not involve large scale molecular motion. In addition, in order to apply these techniques to biological samples such as proteins, preliminary TA spectra measurements were performed on the light-harvesting antenna complex, LH2, of the purple photosynthetic bacterium.
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| Free Research Field |
物理化学
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| Academic Significance and Societal Importance of the Research Achievements |
生物の中には「クリプトビオシス」と呼ばれる驚異的な耐乾燥性や耐凍結性を示すものがある。たとえば、アフリカの砂漠に住むネムリユスリカの幼虫は、新陳代謝が完全に静止した17年間の乾燥状態の後に水を加えると蘇生したとの報告がある。クリプトビオシスの際、糖やその他の物質がガラス転移を起こし、極度に分子運動が抑制され、生体物質の劣化に関与する不要な化学反応を防止すると考えられる。そのメカニズムを解明することは学術的な意義のみではなく、臓器や生体物質の長期保存等の医学的な応用が期待できる研究であり、社会的な意義も大きい。そこで我々は、糖のガラス転移に関して分光学的な実験手法による研究を行ってきた。
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