2023 Fiscal Year Final Research Report
Development of a spheroid containing a water ghost as a pump
| Project/Area Number |
22K18951
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 28:Nano/micro science and related fields
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2022-06-30 – 2024-03-31
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| Keywords | スフェロイド / ポンプ / 拍動 |
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| Outline of Final Research Achievements |
In this study, the aim was to achieve enlargement of spheroids by embedding pumps inside them. Electrically driven pumps were deemed unsuitable due to the necessity of waterproofing and wiring, as well as the difficulty in manufacturing at sizes around a few millimeters. Therefore, hollow particles were utilized as pumps in this study, intended to be embedded in spheroids and driven by pressure in liquid environments. Hollow particles composed of alginate gel, silicone tubes, and polydimethylsiloxane (PDMS) were fabricated, and upon investigation of operational conditions, PDMS was found to be the most suitable material. Additionally, temperature-driven pumps were also considered.
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| Free Research Field |
組織工学、細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
スフェロイドとは細胞をボール状に凝集させた人工組織である。通常のシャーレによる培養よりも高い生理学性を示すため、また、再生医療における組織の最小単位となるため、その重要性はますます高まっている。しかしながら、酸素供給が律速となって、直径200ミクロン程度までが培養可能なサイズとなっていた。本研究ではスフェロイドの中に非接触的・非電気的に駆動できる中空粒子を埋め込み、これを圧力によってポンプのように駆動できることを示した。また、温度変化によって駆動するポンプも検討し、駆動させることによって壊死が抑制できることを明らかとした。これらの成果は、組織工学や再生医療の分野で大きなブレイクスルーである。
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