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2023 Fiscal Year Final Research Report

Development of highly sensitive real-time monitoring biosensors using asynchronous optical sampling picosecond ultrasonics

Research Project

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Project/Area Number 22K18973
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 29:Applied condensed matter physics and related fields
Research InstitutionOsaka University

Principal Investigator

Nagakubo Akira  大阪大学, 大学院工学研究科, 助教 (70751113)

Project Period (FY) 2022-06-30 – 2024-03-31
Keywordsピコ秒超音波法 / 非同期計測 / バイオセンサ / リアルタイムモニタリング / 超音波
Outline of Final Research Achievements

In this study, we developed a highly sensitive, label-free, real-time biosensor using GHz-THz ultrasound by combining an asynchronous picosecond ultrasound method with a nanoscale free-standing thin film. Thermal and acoustic models were constructed to optimize the oscillator and to quantitatively estimate the effect of temperature rise due to laser heating. The oscillator was used to monitor the adsorption reaction of protein A and immunoglobulin G using asynchronous picosecond ultrasound as a model of antigen-antibody reaction, monitoring the change in resonance frequency during the flow or drop of immunoglobulin G solutions at concentrations of 10 and 100 ng/ml, and observing a decrease in resonance frequency of about 1% (=10,000 ppm) at most was successfully detected.

Free Research Field

音響物理学

Academic Significance and Societal Importance of the Research Achievements

バイオセンサの発展はまだまだ探求が続いている。本研究ではその重要な一角を占める質量検出型バイオセンサの可能性について研究した。ナノ振動子を用いることで感度はさらに向上する可能性が高く、さらに非同期ピコ秒超音波法を組み合わせることによりリアルタイムで10-100 GHz帯の共振スペクトルを数秒ごとにモニタリングし続けることができる本手法は非常に重要である。またレーザ光を数ミクロンまで集光した計測法であるためハイスループット化も可能であり、そのためには今後ますますの計測時間の短縮化、SN比の向上、振動子の高周波化など、発展の余地が尽きない。

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Published: 2025-01-30  

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