Cellar differentiation on the electronic semiconductor device induced by the electric stimulation

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K18976
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 29:Applied condensed matter physics and related fields
• Research Institution: Okayama University
• Principal Investigator: Kano Jun 岡山大学, 環境生命自然科学学域, 准教授 (50375408)
• Co-Investigator(Kenkyū-buntansha): 山本 泰生 静岡大学, 情報学部, 准教授 (30550793) ; 竹田 哲也 岡山大学, 医歯薬学域, 研究准教授 (30302368) ; 守屋 央朗 岡山大学, 環境生命科学学域, 准教授 (60500808)
• Project Period (FY): 2022-06-30 – 2024-03-31
• Keywords: 半導体物性 / 分子生物学

Outline of Final Research Achievements

Our project aims to control cell behavior by applying electric fields and currents. DX technology has been introduced because cell behavior and life phenomena are complex systems. We have developed a new electrical stimulation cell that does not adversely affect the cellular growth environment. Our electrical stimulation cells have used carbon plate electrodes attached to a copper-wired circuit on a printed circuit board. Still, there were problems with (1) contamination of the culture medium by copper ions and (2) mold growth due to the pore structure of the carbon electrodes. The shape change was studied in depth from the imaging of cell shape change under electrical stimulation, and the spatial spread between cells was successfully captured at the same time as the shape change caused by the electrical stimulation.

Free Research Field

固体物理学

Academic Significance and Societal Importance of the Research Achievements

新たに開発した電気刺激セルで採用した電極では，表面酸化，純度の問題，特定の有機物の表面固定化，などの問題が生じているが従来の炭素電極に比べずいぶんとハンドリングが改善され，当初抱えていた問題をほぼ解決するに至った。DXにおいても電気刺激強度を大きく変えた系での測定結果を含めた学習を始める準備を進めており，我々のAIがこれまで捉えてきた細胞挙動が真に電気刺激を捉えているのかを判断できるようにしていく。なお新しい2024年度より，本課題で得られた成果を元にした新しいプロジェクトがAMEDで開始されている。