2023 Fiscal Year Final Research Report

Development of plasma injection probe for in vivo and high spacial resolution drug measurement in living organisms

Research Project

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Project/Area Number	22K19044
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 34:Inorganic/coordination chemistry, analytical chemistry, and related fields
Research Institution	Tokyo Institute of Technology
Principal Investigator	Okino Akitoshi 東京工業大学, 科学技術創成研究院, 准教授 (60262276)
Co-Investigator(Kenkyū-buntansha)	高松利寛東京理科大学, 研究推進機構生命医科学研究所, 講師 (10734949) 東海林敦東京薬科大学, 薬学部, 准教授 (90459850) 守岩友紀子東京薬科大学, 薬学部, 助教 (80881515) 八井田朱音東京工業大学, 科学技術創成研究院, 特任助教 (50978608)
Project Period (FY)	2022-06-30 – 2024-03-31
Keywords	大気圧プラズマ / 薬剤分析 / 質量分析 / 注射プラズマプローブ
Outline of Final Research Achievements	A plasma injection probe with a small plasma jet of about 0.70 mm in diameter capable of producing helium plasma was fabricated and integrated into a 16 G, 20 mm injection needle. 15 mm of the plasma injection probe was inserted into a bio-simulated sample of 4-isopropylantipirine homogeneously mixed in agar, and plasma was generated inside the sample. The probe was inserted 15 mm into the sample and plasma was generated inside the sample for mass analysis. As a result, a drug-derived mass signal was confirmed, demonstrating that the drug inside the sample could be analyzed. Next, analysis was performed on a simulated biological sample with different drug concentrations at different depths, and the position resolution in the depth direction was about 0.4 mm. As described above, we succeeded in demonstrating the possibility of mass spectrometry of drugs in living organisms with high spatial resolution and high sensitivity.
Free Research Field	大気圧プラズマ工学
Academic Significance and Societal Importance of the Research Achievements	抗がん剤等の薬剤が十分な効果を発揮するためには，治療対象となる組織に適切な濃度で薬剤が到達する必要がある。しかし現在は，薬剤にRIなどのタグを付与して生体内の分布を測定したり，動物実験で摘出した組織を分析するなどの方法が用いられている。本研究で開発した注射プラズマプローブでは，低侵襲でありながら高空間分解能かつリアルタイムな生体内薬剤分析を実現できる可能性を示せた。また，直径700ミクロンの大気圧低温プラズマジェットを開発して注射針内でプラズマを生成できたため，生体内での焼灼，殺菌，洗浄など他の多くの医療・生命関連応用が期待できる。さらに，超小型プラズマジェットは，工業的にも利用価値が大きい。