Brain-Gut-Microbe Interaction Based on Genetic Structure and Habitat of Plasmalogen-producing Bacteria

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K19155
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 38:Agricultural chemistry and related fields
• Research Institution: Kyushu University
• Principal Investigator: Katsumi Doi 九州大学, 農学研究院, 教授 (40253520)
• Co-Investigator(Kenkyū-buntansha): 本庄 雅則 九州大学, 医学研究院, 教授 (90372747)
• Project Period (FY): 2022-06-30 – 2024-03-31
• Keywords: プラズマローゲン / Lactococcus / Enterococcus / Bifidobacterium / 通性嫌気性 / 絶対嫌気性

Outline of Final Research Achievements

In this study, plasmalogene synthesis genes (plsA) from Lactococcus cremoris, Enterococcus faecalis, and Bifidobacterium longum were introduced into E. coli, and Pls production was measured when the recombinant bacteria were grown under aerobic or anaerobic conditions. The amount of Pls production was measured when the recombinant was cultured under aerobic and anaerobic conditions. E. coli transfected with plsA from facultative anaerobes produced Pls under both conditions, while E. coli transfected with plsA from absolute anaerobes did not produce Pls under aerobic conditions.
When L. cremoris was cultured under various conditions and Pls production was measured, the maximum amount of Pls was detected in M17 medium, 25°C, and anaerobic culture conditions.

Free Research Field

農芸化学

Academic Significance and Societal Importance of the Research Achievements

我々はプラズマローゲン(Pls)の脳認知機能改善に着目し、通性嫌気性の乳酸菌におけるPls探索を行った。その結果、複数株が多様なPls種を生産し、特に腸管常在性乳酸菌株がPlsを高生産する事を認めた。本株由来のPlsの認知機能改善効果を検証するため、Pls生産性増強を試みた。培養条件改変で数倍の生産量増加が達成でき、Pls合成遺伝子の単離と本遺伝子の大腸菌での異種発現によるPls生産に成功した。