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2023 Fiscal Year Final Research Report

Environmental DNA epigenetics using DNA methylation as a marker.

Research Project

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Project/Area Number 22K19346
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionKobe University

Principal Investigator

MINAMOTO Toshifumi  神戸大学, 人間発達環境学研究科, 教授 (50450656)

Co-Investigator(Kenkyū-buntansha) 山中 裕樹  龍谷大学, 先端理工学部, 准教授 (60455227)
Project Period (FY) 2022-06-30 – 2024-03-31
Keywords環境DNA / DNAメチル化 / エピジェネティクス / バイサルファイトシーケンス
Outline of Final Research Achievements

To further develop the environmental DNA analysis method, which is recognised for its usefulness as a simple biomonitoring method, and to sublimate it as a method to determine the state of an organism, such as whether it is parent or offspring, we worked on the development of an ‘environmental DNA epigenetics’ method, which analyses the methylation state of DNA with environmental DNA. The results of experiments using zebrafish showed that, by selecting appropriate genetic markers, the ‘environmental DNA epigenetics’ analysis, which reveals the methylation state of an organism by its environmental DNA, is possible.

Free Research Field

生態学

Academic Significance and Societal Importance of the Research Achievements

生物モニタリングの新手法として有用な環境DNA分析手法を更に発展させ、フィールドの水を汲むだけで生物の生理状態などをモニタリングすることのできる新たな手法の開発に成功した。この手法を用いることで、野生生物がどのような生理状態でいるのか、いつどこで繁殖しているのかなど、従来の環境DNA分析では得ることのできなかった情報を簡便に得られる可能性がある。この手法が発展することで、生物多様性の保全や、水産有用魚種の持続的利用などに応用可能である。

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Published: 2025-01-30  

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