Development of an in vivo chemical labeling method to label cancer-stromal interactions during metastasis

2024 Fiscal Year Final Research Report

• Project/Area Number: 22K19464
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 50:Oncology and related fields
• Research Institution: Kansai Medical University
• Principal Investigator: SAKAMOTO Takeharu 関西医科大学, 医学部, 教授 (70511418)
• Project Period (FY): 2022-06-30 – 2025-03-31
• Keywords: がん / 転移 / ケミカルラベリング

Outline of Final Research Achievements

Mutational PCR for HRP and mgSrtA was performed to construct a transmembrane expression library, and mutants with high labeling efficiency were screened. The mutant was obtained. We transplanted EO771 cells with this mutant into the tail vein and administered Alexa488-LPETG peptide into the tail vein 4 hours after transplantation to see if it could label lung cells in contact with tumor cells such as vascular endothelial cells in vivo in a model of lung metastasis. Unfortunately, the in vivo labeling efficiency was not sufficient to distinguish it from the background.

Free Research Field

分子腫瘍学

Academic Significance and Societal Importance of the Research Achievements

今回の実験では残念ながらin vivoのラベリングに耐えうる変異体は得られなかった。本研究実施時に、SrtAとG5を用いたuLIPSTICの実験系によるin vivoラベリングの手法がNatureに報告されたが、本研究の手法を応用してG5の標識効率を上げるSrtA変異体の開発を行えばより、効率なラベリング手法の開発が期待される。