Development of methods for monitoring metabolic states using stable isotope analysis at natural abundance levels

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K19726
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: YAMADA Keita 東京工業大学, 物質理工学院, 准教授 (70323780)
• Project Period (FY): 2022-06-30 – 2024-03-31
• Keywords: 安定同位体自然存在度分析 / 呼気分析 / 非侵襲代謝モニタリング

Outline of Final Research Achievements

A method was established to analyse the stable carbon isotopic composition of acetone, isoprene and carbon dioxide in exhaled breath at natural abundance levels. The established method was applied to the exhaled breath of three subjects and changes in the concentration and stable isotopic composition of acetone in the exhaled breath were monitored for four days together with changes in blood glucose levels. No correlation was found between blood glucose level changes and exhaled acetone concentration changes, which has been pointed out previously, and it was found that exhaled acetone concentration was not an indicator of blood glucose level changes. A correlation was observed between changes in acetone concentration and changes in stable isotope composition, suggesting that the exhaled acetone concentration reflects the proportion of glucose and lipid metabolism.

Free Research Field

有機地球化学・環境化学

Academic Significance and Societal Importance of the Research Achievements

現在健康科学・医科学分野で用いられる、標識された代謝前駆体の投与と代謝物の同位体標識の程度を追跡する標識同位体技術は明確な代謝情報を提供するが、非常に高価な標識化合物を必要とし、投与量によっては人体に有害となることや、通常とは異なる代謝状態を形成する可能性がある。さらに、標識化合物の滞留時間のみの限られた代謝状態の観測となり、長期モニタリングは出来ない。標識化合物の投与を必要とせず、通常の代謝状態変化を長期間モニタリングが可能となる安定同位体自然存在度技術を、健康科学・医科学分野に導入したことが本研究の意義である。