2023 Fiscal Year Final Research Report
Combination of fluorescence and scattered light measurements for rapid determination of cell-derived microparticles.
| Project/Area Number |
22K19886
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
Kinjo Masataka 北海道大学, 先端生命科学研究院, 名誉教授 (70177971)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
北村 朗 北海道大学, 先端生命科学研究院, 講師 (10580152)
|
|---|
| Project Period (FY) |
2022-06-30 – 2024-03-31
|
| Keywords | 蛍光測定 / 光散乱測定 / 相互相関関数 / 微粒子 / 蛍光微粒子 |
|---|
| Outline of Final Research Achievements |
Membrane-free organelles (membraneless organelles ) and protein condensates based on liquid-liquid phase separation phenomena have been considered as transmitters of dementia and neurodegenerative diseases. When fluorescence-labelled antibodies are used for their detection, particles with the corresponding surface antigens can be detected with high sensitivity, but others remain undetected and invisible. Scattering, on the other hand, gives a profile of the whole particle, but does not allow determination of particles with specific functions
The Principal Investigator focused on the fact that Fluorescence Correlation Spectroscopy (FCS), like light scattering, is a method that reflects the dynamic properties derived from the diffusive motion of particles and molecules, and investigated the possibility of combining scattering and fluorescence measurements. The possibility of combining scattering and fluorescence measurements was studied.
|
| Free Research Field |
生物物理
|
| Academic Significance and Societal Importance of the Research Achievements |
生体由来の微粒子やタンパク質凝集体の測定方法には大きく蛍光測定と散乱測定がある。蛍光測定は特異性が高いが微弱光測定であり，試料や測定に繊細な調整が必要である。一方，散乱測定は特異性は低いものの，散乱光シグナルは蛍光比較して強く検出はやや容易であり，多くの粒子の情報を得ることが可能である。この二つの同時測定を融合することで，特異的なシグナルと網羅的な情報を得ることで，新規の生体微粒子解析法の開発を目指した。両方測定可能な励起光を見つけることは困難であったが，同じ装置の光路で測定が可能であることは示すことができた。今後，試料の調整条件などを検討することで，同時測定が達成されると考える。
|
