2023 Fiscal Year Final Research Report
Development of liver regeneration therapy using super exosomes with multiple and high volume protein delivery systems
| Project/Area Number |
22K19933
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐野 将之 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (80415687)
西村 健 筑波大学, 医学医療系, 教授 (80500610)
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| Project Period (FY) |
2022-06-30 – 2024-03-31
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| Keywords | エクソソーム / 間葉系幹細胞 |
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| Outline of Final Research Achievements |
In this study, we succeeded in introducing tdTomato and Annexin A1 into exosomes, and confirmed that tdTomato is taken up by macrophages in large amounts in cirrhosis, providing basic data on the dynamics of exosomes for therapeutic purposes. In addition, the introduction of Annexin A1, which we consider to be one of the potential factors that may lead macrophages to become anti-inflammatory, was confirmed, but did not lead to a dramatic increase in therapeutic efficacy. Although there are issues to be addressed in the future, such as the possibility that a single protein was not sufficient and that the overall contents may have been altered, the establishment of the introduction system was very significant and provided fundamental data for the future.
Translated with DeepL.com (free version)
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| Free Research Field |
肝臓病学
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| Academic Significance and Societal Importance of the Research Achievements |
エクソソームは細胞培養上清から修飾を施さずにそのまま採取される天然型や特定の細胞株培養上清液や特定の動植物やその培養細胞などからエクソソームを精製・回収した後にペプチド付与などの修飾を施したり、エクソソームの供給元である細胞あるいは動植物等に遺伝子改変を施したりすることで、特定の機能をEVsに付与するもの、また特定の治療薬を内包することでドラッグデリバリーシステム（DDS）の担体として利用する改変型EVsがあり、今回の結果は、改変型エクソソームを将来、目指す上での重要な基盤データを得ることができた。
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