2022 Fiscal Year Research-status Report
Production of 2-D nanocarbons with different bioaffinities as ROS quenching agents to improve mammalian oocyte cryopreservation outcomes
| Project/Area Number |
22K20535
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| Research Institution | Okayama University |
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Principal Investigator |
FERREPUJOL PILAR 岡山大学, 異分野融合先端研究コア, 特任助教 (50839008)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | graphene oxide / reduced graphene oxide / oocyte / in vitro maturation |
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| Outline of Annual Research Achievements |
We prepared graphene oxide (GO) according to a previous method (Hummer’s method) and slowly performed thermal reduction at 80°C up to 2 weeks to ensure a successful reduction of the functional groups to obtain reduced graphene oxide (rGO). The GO and rGO samples were structurally analyzed by FTIR, TGA, XPS and XRD. To analyze their reactive oxygen species (ROS) activity, they were analyzed by ESR. Furthermore, to understand their ability to be used in a culture medium setting, the samples were submitted to a dispersion test.
According to the results of all the tests, pristine GO (oxidation level 30-35%) was selected to analyze its toxicity during oocyte in vitro maturation (IVM) in the presence of non-enzymatic antioxidants. Results suggest that the addition of GO during oocyte IVM does not negatively affect oocyte viability significantly.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have finalized stage one of our research, related to the preparation and analysis of GO and rGO. Furthermore, we analyzed the safety to use this material in an in vitro setting.
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| Strategy for Future Research Activity |
We intend to further analyze the effect of GO on oocyte IVM in the absence of antioxidants, to assess its use as a ROS quencher to protect the oocytes in an in vitro setting from oxidative stress.
Depending on the results of the experiment, we are also planning to assess the effect of GO addition during oocyte vitrification, as well as after oocyte vitrification, during the recovery stage, to see if it can reduce oocyte damage by reactive oxygen species (ROS) after thawing.
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