2023 Fiscal Year Annual Research Report

Production of 2-D nanocarbons with different bioaffinities as ROS quenching agents to improve mammalian oocyte cryopreservation outcomes

Research Project

Project/Area Number	22K20535
Research Institution	Okayama University
Principal Investigator	FERREPUJOL PILAR 岡山大学, 異分野融合先端研究コア, 特任助教 (50839008)
Project Period (FY)	2022-08-31 – 2024-03-31
Keywords	graphene oxide / reduced grapehene oxide / cryoprotectant / oocyte / vitrification
Outline of Annual Research Achievements	The results indicated that when graphene oxide (GO) and reduced graphene oxide (rGO) were used during vitrification, they negatively affected the survival of cumulus-oocyte-complexes due to the strong affinity of the materials with the cell membranes. Furthermore, when the materials were added after thawing, their protective effect against reactive oxygen species could also not be clearly evaluated. The high reactivity of the surface of GO with the inorganic components of the culture media (especially with positive ions such as Na, K or Ca) could partly explain the reason why pristine GO and rGO were unable to successfully protect the oocytes from oxidative stress. Further studies employing surface-modified GO are necessary to successfully use this material for cryopreservation.

(1 results)