Functional analysis of septin and its regulators in ascospore development of phytopathogenic filamentous fungi

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20579
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0602:Agricultural and environmental biology and related fields
• Research Institution: Kyoto University
• Principal Investigator: Tsuji Kenya 京都大学, 農学研究科, 特定助教 (10965940)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: 植物病原菌 / 有性生殖 / 子嚢胞子 / MSUD / セプチン

Outline of Final Research Achievements

In a previous study, the localization analysis method using fluorescent protein in the asci of the phytopathogenic filamentous fungi Bipolaris maydis has been established. In this study, we also found that eisosome protein is essential for the ascospore formation and that the localization pattern of eisosome protein is similar to that of core septin Cdc10 based on fluorescence observation. In addition, SNARE protein and phospholipase D were found to be essential for normal ascospore formation. We also showed that the structure of core septin Cdc10 may change during vegetative growth and during ascospore formation.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

本研究では病原糸状子嚢菌の有性生殖における子嚢の発達から子嚢胞子形成までの段階的なRNA-seq解析を実施した。このデータは子嚢胞子の発生メカニズムを理解するための重要な手掛かりになると考えている。また、植物病原菌にとって有性生殖は新たな薬剤抵抗性および病原力の獲得のきっかけとなるだけでなく、主要な分散減として機能している場合も存在する。そのため、新たな植物防除法の開発にも寄与すると考えている。