Molecular mechanisms of resistance genes from wheat and barley recognizing the blast fungus

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20580
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0602:Agricultural and environmental biology and related fields
• Research Institution: Kobe University
• Principal Investigator: Asuke Soichiro 神戸大学, 農学研究科, 助教 (90882514)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: いもち病菌 / 抵抗性遺伝子 / オオムギ / コムギ

Outline of Final Research Achievements

The barley blast resistance gene Rmo2 and the wheat resistance gene Rwt7 recognize the avirulence genes PBY2 and PWT7, respectively. It was hypothesized that the integrated domain at the N-terminus of Rmo2 and Rwt7 plays an important role in effector recognition. Using protoplast assays with domain swap constructs, it was revealed that the integrated domain of Rmo2 specifically recognizes PBY2, while the integrated domain of Rwt7 specifically recognizes PWT7. Additionally, by screening the effectors of rice and foxtail millet blast fungi based on the three-dimensional structure, we successfully identified an avirulence effector gene recognized by Rmo2.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

NBS-LRR型抵抗性遺伝子に見られるintegrated domainによるエフェクター認識メカニズムが明らかにされる中、本研究では、protein kinase型抵抗性遺伝子における同ドメインのエフェクター認識機構の一端を解明した。
その結果、統合ドメインによる認識拡張が、分子種に依存せず抵抗性遺伝子の分子機能進化において重要な役割を果たすことが示唆された。
この認識機構を応用することで、新たなエフェクターを標的としたムギ類の分子抵抗性育種技術の開発が期待される。