2023 Fiscal Year Final Research Report
Identification of gene involved in heartwood norlignan biosynthesis in conifers.
| Project/Area Number |
22K20593
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0603:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YAMAMURA Masaomi 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授 (00948367)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | 心材形成 / 遺伝子発現解析 |
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| Outline of Final Research Achievements |
In this study, we established methods of crude enzyme preparation and total RNA extraction for each part of Japanese cedar (heartwood, transition wood, sapwood, leaves, and branch) in order to identify genes involved in the biosynthesis of norlignans that show antimicrobial activity and are produced at the same time as heartwood formation in cedar.
In the RNA-seq analysis, we succeeded in obtaining a small amount of data on expressed genes even in the heartwood, which is generally known as dead tissue. Liquid suspension cultureof japanese cedar callus were also successfully prepared and are ready for future RNA-seq analysis of their callus.
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| Free Research Field |
木質科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得たスギの心材、移行材、辺材部でのRNA-seqの成果は、スギ心材がどのように形成されるかという長年の謎を、分子生物学的観点から明らかにする第一歩の情報を提供するという点において学術的意義があり、本研究は将来的には心材成分のさらなる増強などにより高付加価値化されたスギ材が創出でき得るという社会的意義がある。
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