New Survey Method of Aquatic Organisms in Paddy Areas by Immunoassay

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20605
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0604:Agricultural economics and rural sociology, agricultural engineering, and related fields
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Watabe Keiji 国立研究開発法人農業・食品産業技術総合研究機構, 農村工学研究部門, 上級研究員 (50527017)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: イムノアッセイ / 田んぼの生きもの調査 / 水生生物 / 農業水路

Outline of Final Research Achievements

A method for detecting proteins derived from aquatic organisms contained in the water of paddy areas (paddy fields, agricultural canals, reservoirs etc.) were investigated. The presence or absence of reactions between several species of aquatic organisms and immunochromatographic kits for fish and crustaceans were clarified, and it is demonstrated that the detection kits are possible to detect up to about 10 ppm by testing at different dilution ratios. In addition, it is demonstrated that antibodies produced based on marine mussel larvae can detect golden mussels (Limnoperna fortune) using the dot blot method and whole-mount immunostaining method, and a prototype immunochromatographic detection kit capable of detecting the golden mussels was developed.

Free Research Field

農村環境工学

Academic Significance and Societal Importance of the Research Achievements

特定外来生物に指定されるカワヒバリガイの早期検出に向けて、本成果は、現地で簡易に検出できる方法として応用が期待される。これに向けて、感度の向上や種特異性の検討、サンプリング方法の最適化などが更なる課題として挙げられる。
魚類検出キットおよび甲殻類検出キットは、水田水域で採食する高次捕食者（トキやコウノトリ、サギ類等）の餌資源の指標として活用しうると考えられる。