Construction of an eight-segment influenza D virus with M1 and M2 genes.

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20619
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0605:Veterinary medical science, animal science, and related fields
• Research Institution: Azabu University
• Principal Investigator: Ishida Hiroho 麻布大学, 獣医学部, 助教 (40963476)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: D型インフルエンザウイルス / インフルエンザウイルス / オルソミクソウイルス / 牛呼吸器病症候群 / リバースジェネティクス / M1 / P42 / M2

Outline of Final Research Achievements

Influenza D virus (IDV) is considered to be one of the causative viruses of bovine respiratory disease complex. In this study, an eight-segmented IDV with M1 and M2 (p42) genes in separate segments was successfully generated by reverse genetics. We also attempted to generate a partial deletion virus with M1 and M2 (p42) proteins deleted by 10 amino acids each. The results revealed that six of the amino acid regions common to both M1 and M2 (p42) proteins have important functions in the M1 protein while not essential functions in the p42 protein.

Free Research Field

獣医学

Academic Significance and Societal Importance of the Research Achievements

本研究では、①M1とM2(p42)遺伝子をそれぞれ別の分節(M1とM2分節)にもつ8分節型IDVの作出法を確立した。本手法は、M1・M2タンパク質の機能の個別解析を可能にするため、IDVの分子生物学的解析の新しい基盤に成り得ると期待される。また、それぞれのタンパク質に独立した変異の導入を可能にするため、M1・M2タンパク質を標的にした弱毒生ワクチン開発への応用も期待される。