Studying bone secreted molecules (osteokines) as a novel diabetes diagnostic tool

2023 Fiscal Year Annual Research Report

• Project/Area Number: 22K20647
• Research Institution: Tohoku University
• Principal Investigator: MARAHLEH ASEEL・MAHMOUD・SULEIMAN 東北大学, 学際科学フロンティア研究所, 助教 (10963100)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: Osteocytes / Transcriptome / Proteome / Glut 1 / Diabetes / Bone / Osteokines

Outline of Annual Research Achievements

The purpose of this research is two-fold: 1.Evaluate the regulation of energy homeostasis by the skeleton (bone) and 2.The effect of energy perturbation on the skeleton. We developed Glut1 bone specific knockout mice to study the effect of glucose abstinence on bone structural and functional parameters. Mice body weight decreased in the knockout group which indicates that glucose metabolism by the skeleton affects whole body metabolism and bone density. Additionally transcriptome and proteome-wide analysis conducted on hyperglycemic osteocytes (bone cells) were used to constructing a molecular network to reveal a bone-specific effect of hyperglycemia across the analyzed omics levels which revealed a dissociation between transcription (gene expression) and translation (osteokine levels).

Research Products

• [Presentation] The transcriptomic landscape of hyperglycemic osteocytes. (2023)
  • Author(s): Marahleh A, Rashad S, Kitaura H, Jiayi R, Ohori F, Noguchi T, Mizoguchi I
  • Organizer: The 82nd Annual Meeting of the Japanese Orthodontic Society.
• [Presentation] Hyperglycemia Induces Extensive Alternative Splicing Changes In Osteocyte Enriched Cultures With Minimal Transcriptional Alterations. (2023)
  • Author(s): Marahleh A, Rashad S, Kitaura H, Jiayi R, Ohori F, Noguchi T, Mizoguchi I
  • Organizer: ASBMR Pre-Meeting Symposium on Osteocytes in Bone Health and Disease and as Therapeutic Target Cells. The American Society of Bone and Mineral Research
  • Int'l Joint Research
• [Presentation] Osteoimmunology in Orthodontics (2023)
  • Author(s): Aseel Marahleh
  • Organizer: STOVIT online series, Universitas Airlangga,
  • Invited
• [Presentation] High Glucose Induces Alternative Splicing Changes in Primary Osteocytes With Minimal Transcriptional Alterations. (2023)
  • Author(s): Marahleh A, Rashad S, Kitaura H, Jiayi R, Ohori F, Noguchi T, Mizoguchi I
  • Organizer: Cell Bio 2023. Boston Convention Center, Boston, MA, USA., The American Society for Cell Biology
  • Int'l Joint Research
• [Presentation] Osteocytes Exhibit Extensive Alternative Splicing Changes in Response to Hyperglycemia. (2023)
  • Author(s): Marahleh A, Rashad S, Kitaura H, Jiayi R, Ohori F, Noguchi T, Mizoguchi I
  • Organizer: The 46th Annual Meeting of the Molecular Biology Society of Japan. Online.
• [Presentation] Metabolism and the Skeleton. What can our bones tell us? (2023)
  • Author(s): Aseel Marahleh
  • Organizer: 2022 CA+inD Winter Short-term Exchange Program Multimodal Global Leaders Development through Asian-Model Dentistry Consortium
  • Invited