2023 Fiscal Year Final Research Report
Effect of extracellular matrix from decellularized tissues on differentiation and migration of enteric nerve stem cells
| Project/Area Number |
22K20655
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0702:Biology at cellular to organismal levels, and related fields
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | 再生医療 / 組織工学 / 脱細胞 / ヒルシュスプルング病 / 神経幹細胞 / 腸管神経節 / 小腸機能不全 |
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| Outline of Final Research Achievements |
This study aims to elucidate the causes of Hirschsprung's disease and develop new treatments. We collected tissue samples from both healthy and diseased parts of intestines removed during surgery and removed the cells through a special chemical process (decellularization). This process effectively removed DNA while preserving the intestinal extracellular matrix (ECM). We are using this ECM as a platform for stem cell culture, with the potential to contribute to future studies of neural stem cells and the development of new treatments.
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| Free Research Field |
再生医療
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| Academic Significance and Societal Importance of the Research Achievements |
ヒルシュスプルング病は先天的な病気であり、腸管の発生過程で腸管神経幹細胞の遊走が停止することにより腸管神経節が欠如する病気ですが、遊走が呈する原因は明らかになっていません。現在の治療法は異常な腸管を切除する手術治療のみでありますが、病変が広範囲に及ぶ症例では腸管の大量切除に伴う合併症が問題になります。本研究ではヒルシュスプルング病の発症とECMの関連性に注目し、その原因を究明することで手術以外の治療選択肢を開発することを目的としております。
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