2023 Fiscal Year Final Research Report
Inflammation/anti-inflammatory regulation by lateral parabrachial nucleus
| Project/Area Number |
22K20699
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0704:Neuroscience, brain sciences, and related fields
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | 外側腕傍核 / カルシウムイメージング / 炎症制御 |
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| Outline of Final Research Achievements |
We established an experimental system to measure neural activity in the lateral parabrachial nucleus (LPB) in real-time in awake, unrestrained animals using Ca2+ imaging. The results suggested that inflammatory and anti-inflammatory substances might differently affect Ca2+ dynamics in the LPB. As the LPB is molecularly and functionally heterogeneous, these phenomena might be due to differences in observed neural populations. Therefore, we aimed to observe and manipulate LPB neurons active during inflammatory or anti-inflammatory responses using activity-dependent promoter. However, unwanted labeling occurred due to the restraint and injection, as the LPB is strongly activated by pain and fear stimuli. Although non-specific labeling was significantly reduced after optimizing conditions, the process took longer than expected, and we did not fully achieve the initial plan.
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| Free Research Field |
神経化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではLPBが全身の炎症反応/抗炎症反応に応じて異なるCa2+動態を示すことを報告した．疼痛関連領域であるLPBが炎症状態のみならず，抗炎症状態までも感知するという事実はこれまで知られておらず，中枢神経系を介した新しい免疫系への介入戦略を構築する上で重要な意味をもつと期待される．また，LPBは痛みに加え，呼吸・循環・体温・味覚等の多岐にわたる感覚情報の入力を受ける場であることから，本研究で確立を試みたLPBの標識技術は他領域の研究においても活用できる技術である．
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