2022 Fiscal Year Research-status Report
Exploring the exosome-nanosome combinatorial platform as a novel, universal, and ideal nanoparticle-based drug delivery system
| Project/Area Number |
22K20705
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| Research Institution | Hokkaido University |
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Principal Investigator |
タン ロジャー・サルバシオン 北海道大学, 先端生命科学研究院, 助教 (10951759)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | Exosome-nanosme / Exosome / Cancer cells / Nanoparticle / Nanosome |
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| Outline of Annual Research Achievements |
During the first fiscal year of research implementation, production and isolation have been optimized to yield high number of exosomes. This part is important for the incorporation of "nanosome" as the number of "exosome-nanosome" platform to be produced is dependent on the produced exosomes. The amount of exosome produced between fed and starved cells were also determined to be different. Some exosome proteins are downregulated in the starved state. These are significantly important as the "exosome-nanosome" incorporation on different cells/organs can also be impacted significantly.
The construction of the platform showed limitations on the incorporation of the "nanosome" and optimization (sizes, ligand composition, and the overall charge) seemed to be necessary.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
External Factor (Uncontrolled):
Reagent purchasing challenges were encountered. There are reagent, antibodies, gels, filters, and column, that are not available in Japan and have to be imported from abroad. Delivery of these reagents usually takes 4-6 weeks.
Internal Factor (Controlled):
During the implementation, challenges and hurdles that require optimization
steps were encountered. These optimization trials for one parameter usually takes a minimum of 2-3 weeks to complete.
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| Strategy for Future Research Activity |
To mitigate the problems on longer delivery time of reagents, bulk ordering will be followed. The next steps will be finishing optimizations, especially on the construction of the platform. After the construction, biodistribution assays will follow, and then drug loading and efficiency testing. To push forward the progress of the research, collaboration (at least personal level) will be sought as well.
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| Causes of Carryover |
This will be used to purchase used up reagents and disposable items.
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