Research for development mechanisms and therapeutic targets of gastric cancer mediated by CHAC1

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20755
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0803:Pathology, infection/immunology, and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: WADA YURIKO 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (60963923)
• Project Period (FY): 2022-03-01 – 2024-03-31
• Keywords: CHAC1 / 胃癌

Outline of Final Research Achievements

Increased expression of CHAC1 leads to the depletion of glutathione and induces oxidative stress, which is thought to possibilities for development and progression of cancer. Although clinicopathological studies have been conducted on the expression of CHAC1 in various cancer types, function of CHAC1 are still unknown.
In this study, we performed clinicopathological analysis with immunohistochemistry on surgical resection specimens of gastric cancer. As a result, immunohistochemical CHAC1 expression was statistically related with lymph node metastasis and also high expression of CHAC1 was correlated with poor prognosis. In addition, we succeeded in creating AGS cells that constitutively CHAC1 was overexpressed by using viral vectors and now we are proceeding with RNAseq analysis in order to investigate induced genes related with CHAC1 overexpression.

Free Research Field

病理

Academic Significance and Societal Importance of the Research Achievements

日本での胃癌罹患率は諸外国と比べ顕著に高く、その要因については多くの研究がされている。CHAC1は本研究室での長年の研究テーマであり、先行研究にてピロリ菌感染が原因となる胃癌はC H AC１の高発現が誘導されることがわかった。本研究では胃癌患者の症例を用い、臨床病理学的解析を行った結果、C H A C１発現はリンパ節転移と相関することが示され、さらにC H A C１の発現が強い胃癌症例ほど予後不良になることがわかった。また、RNAseq解析のため、C H A C１を高発現した培養細胞の作成に成功しており、C H A C１発現により誘導される詳細な遺伝子群の解析を現在進めている。